Cloning of the gene coding for chitobiase of Serratia marcescens

Hadar Kless, Yaron Sitrit, Ilan Chet, Amos B. Oppenheim

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A λ phage DNA library of Serratia marcescens was constructed and a clone carrying the gene coding for chitobiase (E.C.3.2.1.29) was isolated and characterized. Deletion analysis limited the cloned region to 4.5 kb that is capable of efficient expression of chitobiase. Escherichia coli cells harboring a plasmid carrying the cloned gene express chitobiase constitutively. The molecular weight of the protein is about 95000 daltons. In exponentially growing E. coli cells the chitobiase enzyme was found to be secreted into the periplasm.

Original languageEnglish
Pages (from-to)471-473
Number of pages3
JournalMolecular Genetics and Genomics
Volume217
Issue number2-3
DOIs
StatePublished - 1 Jun 1989
Externally publishedYes

Keywords

  • Chitobiase
  • Serratia marcescens

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