Abstract
A λ phage DNA library of Serratia marcescens was constructed and a clone carrying the gene coding for chitobiase (E.C.3.2.1.29) was isolated and characterized. Deletion analysis limited the cloned region to 4.5 kb that is capable of efficient expression of chitobiase. Escherichia coli cells harboring a plasmid carrying the cloned gene express chitobiase constitutively. The molecular weight of the protein is about 95000 daltons. In exponentially growing E. coli cells the chitobiase enzyme was found to be secreted into the periplasm.
Original language | English |
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Pages (from-to) | 471-473 |
Number of pages | 3 |
Journal | Molecular Genetics and Genomics |
Volume | 217 |
Issue number | 2-3 |
DOIs | |
State | Published - 1 Jun 1989 |
Externally published | Yes |
Keywords
- Chitobiase
- Serratia marcescens
ASJC Scopus subject areas
- Genetics