Abstract
A method for the construction of a highly stable enzyme electrode is described. A new mediator, cobalt phthalocyanine, a biocompatible material, was immobilized together with the enzyme glucose oxidase in a colloidal graphite emulsion matrix. The resulting glucose electrode was characterized. The electrode retains its full activity for long periods (more than 11 months). The response to glucose is high, fast and independent of ph (5-8) or oxygen partial pressure. The linear range obtained (0-5 mM) can be extended by the introduction of a dialysis membrane and can thus be adjustedto the measurement of glucose concentrations in the range of medical interest. The electrode was tested successfully on serum samples.
| Original language | English |
|---|---|
| Pages (from-to) | 327-333 |
| Number of pages | 7 |
| Journal | Analytica Chimica Acta |
| Volume | 281 |
| Issue number | 2 |
| DOIs | |
| State | Published - 10 Sep 1993 |
| Externally published | Yes |
Keywords
- Amperometry
- Cobalt phthalocyanine
- Enzymatic methods
- Enzyme electrodes
- Glucose
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy