Color fingerprinting of proteins by calixarenes embedded in lipid/polydiacetylene vesicles

Sofiya Kolusheva, Reza Zadmard, Thomas Schrader, Raz Jelinek

Research output: Contribution to journalArticlepeer-review

117 Scopus citations

Abstract

"Naked eye" color detection of proteins was achieved by embedding calixarene receptors within vesicles comprising phospholipids and the chromatic polymer polydiacetylene. Dramatic visible absorbance changes were induced through electrostatic interactions between the protein surface and the vesicle-incorporated hosts. The colorimetric responses could be induced by micromolar protein concentrations, and furthermore, specific protein fingerprints could be obtained by incorporating different receptors within the vesicles. Fluorescence and circular dichroism experiments confirmed the relationship between the colorimetric phenomena and protein docking on the surface of the chromatic vesicles. The colorimetric assay constitutes a generic platform for high-sensitivity detection of soluble proteins and for evaluation of protein surface charge distribution.

Original languageEnglish
Pages (from-to)13592-13598
Number of pages7
JournalJournal of the American Chemical Society
Volume128
Issue number41
DOIs
StatePublished - 18 Oct 2006

ASJC Scopus subject areas

  • Catalysis
  • Chemistry (all)
  • Biochemistry
  • Colloid and Surface Chemistry

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