Colorimetric Enzyme Sensing Assays via In Situ Synthesis of Gold Nanoparticles

Dnyaneshwar B. Rasale; Indrajit Maity; Apurba K. Das

doi:10.1007/s10876-013-0606-z

Colorimetric Enzyme Sensing Assays via In Situ Synthesis of Gold Nanoparticles

Dnyaneshwar B. Rasale, Indrajit Maity, Apurba K. Das

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We report a successful facile and novel approach for in situ synthesis of gold nanoparticles (AuNPs) via enzymatic dephosphorylation reaction at room temperature. Fmoc-tyrosine phosphate and cytidine-5-mono phosphate are used to sense the activities of an enzyme alkaline phosphatase. Formation of AuNps is highly selective towards biomolecules and it is readily detected colorimetrically and UV-Vis analysis. In this procedure, dephosphorylated product plays both roles as reducing and stabilizing agent to direct the formation of AuNPs in aqueous media. Transmission electron microscopic study reveales that hexagonal AuNPs were synthesized by using Fmoc-tyrosine phosphate and alkaline phosphatase. Wide angle X-ray scattering data confirms the formation of AuNPs. FT-IR studies confirm that biomolecules play crucial role to stabilize the AuNPs by molecular interactions with the surface of AuNPs. In situ synthesized AuNPs are applied for the sensing of enzyme activity.

Original language	English
Pages (from-to)	1163-1170
Number of pages	8
Journal	Journal of Cluster Science
Volume	24
Issue number	4
DOIs	https://doi.org/10.1007/s10876-013-0606-z
State	Published - 1 Dec 2013
Externally published	Yes

Keywords

Enzyme sensing
Gold nanoparticles
In situ synthesis

ASJC Scopus subject areas

Biochemistry
General Chemistry
General Materials Science
Condensed Matter Physics

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10.1007/s10876-013-0606-z

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title = "Colorimetric Enzyme Sensing Assays via In Situ Synthesis of Gold Nanoparticles",

abstract = "We report a successful facile and novel approach for in situ synthesis of gold nanoparticles (AuNPs) via enzymatic dephosphorylation reaction at room temperature. Fmoc-tyrosine phosphate and cytidine-5-mono phosphate are used to sense the activities of an enzyme alkaline phosphatase. Formation of AuNps is highly selective towards biomolecules and it is readily detected colorimetrically and UV-Vis analysis. In this procedure, dephosphorylated product plays both roles as reducing and stabilizing agent to direct the formation of AuNPs in aqueous media. Transmission electron microscopic study reveales that hexagonal AuNPs were synthesized by using Fmoc-tyrosine phosphate and alkaline phosphatase. Wide angle X-ray scattering data confirms the formation of AuNPs. FT-IR studies confirm that biomolecules play crucial role to stabilize the AuNPs by molecular interactions with the surface of AuNPs. In situ synthesized AuNPs are applied for the sensing of enzyme activity.",

keywords = "Enzyme sensing, Gold nanoparticles, In situ synthesis",

author = "Rasale, {Dnyaneshwar B.} and Indrajit Maity and Das, {Apurba K.}",

note = "Funding Information: Acknowledgments The authors are thankful to Department of Science and Technology (Grant No. SR/ FT/CS-67/2010) for financial support. DBR and IM are thankful to CSIR for their fellowship. We thank SAIF IIT Bombay for the use of TEM, UGC-DAE Consortium Indore for the use of powder XRD.",

year = "2013",

month = dec,

day = "1",

doi = "10.1007/s10876-013-0606-z",

language = "English",

volume = "24",

pages = "1163--1170",

journal = "Journal of Cluster Science",

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T1 - Colorimetric Enzyme Sensing Assays via In Situ Synthesis of Gold Nanoparticles

AU - Rasale, Dnyaneshwar B.

AU - Maity, Indrajit

AU - Das, Apurba K.

N1 - Funding Information: Acknowledgments The authors are thankful to Department of Science and Technology (Grant No. SR/ FT/CS-67/2010) for financial support. DBR and IM are thankful to CSIR for their fellowship. We thank SAIF IIT Bombay for the use of TEM, UGC-DAE Consortium Indore for the use of powder XRD.

PY - 2013/12/1

Y1 - 2013/12/1

N2 - We report a successful facile and novel approach for in situ synthesis of gold nanoparticles (AuNPs) via enzymatic dephosphorylation reaction at room temperature. Fmoc-tyrosine phosphate and cytidine-5-mono phosphate are used to sense the activities of an enzyme alkaline phosphatase. Formation of AuNps is highly selective towards biomolecules and it is readily detected colorimetrically and UV-Vis analysis. In this procedure, dephosphorylated product plays both roles as reducing and stabilizing agent to direct the formation of AuNPs in aqueous media. Transmission electron microscopic study reveales that hexagonal AuNPs were synthesized by using Fmoc-tyrosine phosphate and alkaline phosphatase. Wide angle X-ray scattering data confirms the formation of AuNPs. FT-IR studies confirm that biomolecules play crucial role to stabilize the AuNPs by molecular interactions with the surface of AuNPs. In situ synthesized AuNPs are applied for the sensing of enzyme activity.

AB - We report a successful facile and novel approach for in situ synthesis of gold nanoparticles (AuNPs) via enzymatic dephosphorylation reaction at room temperature. Fmoc-tyrosine phosphate and cytidine-5-mono phosphate are used to sense the activities of an enzyme alkaline phosphatase. Formation of AuNps is highly selective towards biomolecules and it is readily detected colorimetrically and UV-Vis analysis. In this procedure, dephosphorylated product plays both roles as reducing and stabilizing agent to direct the formation of AuNPs in aqueous media. Transmission electron microscopic study reveales that hexagonal AuNPs were synthesized by using Fmoc-tyrosine phosphate and alkaline phosphatase. Wide angle X-ray scattering data confirms the formation of AuNPs. FT-IR studies confirm that biomolecules play crucial role to stabilize the AuNPs by molecular interactions with the surface of AuNPs. In situ synthesized AuNPs are applied for the sensing of enzyme activity.

KW - Enzyme sensing

KW - Gold nanoparticles

KW - In situ synthesis

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U2 - 10.1007/s10876-013-0606-z

DO - 10.1007/s10876-013-0606-z

M3 - Article

AN - SCOPUS:84891664053

SN - 1040-7278

VL - 24

SP - 1163

EP - 1170

JO - Journal of Cluster Science

JF - Journal of Cluster Science

IS - 4

ER -

Colorimetric Enzyme Sensing Assays via In Situ Synthesis of Gold Nanoparticles

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Keywords

ASJC Scopus subject areas

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