TY - JOUR
T1 - Combination of N-acetyl Cysteine and Thymoquinone Alleviates Hepatocellular Toxicities by Radiation and CClIntoxication in SD Rats
AU - Zhang, Shu
AU - Chen, Shaohua
AU - Wang, Jingyi
AU - Sun, Qingyu
AU - Alahmadi, Tahani Awad
AU - Natarajan, Nandakumar
N1 - Publisher Copyright:
© Author (s) 2023 Distributed under Creative Commons CC-BY 4.0.
PY - 2023/7/1
Y1 - 2023/7/1
N2 - Radiation and chemicals were the major clinical toxicants known to cause cellular damage during prognostic interventions in vivo. Cellular and molecular damages in the liver were the major causes for the hepatocellular injury due to various toxicities. Though many antioxidants alleviate various types of hepatotoxicities, protection exerted by the combination of N-acetyl cysteine (NA) and Thymoquinone (TQ) in the combined toxicities of radiation and carbon tetrachloride (CCLR) in Sprague-Dawley (SD) rats were unknown. Current research was focused on the protective efficacy of combination of NA with optimized dose of TQ (NATQ) in radiation/CCl4 (CCLR)-induced hepatotoxicities. At the end of the experimental period (6 weeks), body weight, liver weight, serum and liver tissues were analyzed for marker enzymes (AST, ALT, LDH), oxidative stress level (MDA, GSSG), antioxidant status (GSH, Vitamin E, Vitamin C), activities of enzymatic antioxidants (SOD, GPx, CAT, GST), liver histopathology and studies for the hepatic levels of NfkB, IL-6, TNF-α, MMP-3, MMP-12, Nrf2 and HO-1 were done. Significant (p≤0.05) toxicological alterations in the above parameters were recovered to normal in the treatment of NATQ combinations in SD rats. In conclusion, we provide evidence of protective efficacy of NATQ combination in alleviating the hepatotoxicities produced by CCLR in SD rats.
AB - Radiation and chemicals were the major clinical toxicants known to cause cellular damage during prognostic interventions in vivo. Cellular and molecular damages in the liver were the major causes for the hepatocellular injury due to various toxicities. Though many antioxidants alleviate various types of hepatotoxicities, protection exerted by the combination of N-acetyl cysteine (NA) and Thymoquinone (TQ) in the combined toxicities of radiation and carbon tetrachloride (CCLR) in Sprague-Dawley (SD) rats were unknown. Current research was focused on the protective efficacy of combination of NA with optimized dose of TQ (NATQ) in radiation/CCl4 (CCLR)-induced hepatotoxicities. At the end of the experimental period (6 weeks), body weight, liver weight, serum and liver tissues were analyzed for marker enzymes (AST, ALT, LDH), oxidative stress level (MDA, GSSG), antioxidant status (GSH, Vitamin E, Vitamin C), activities of enzymatic antioxidants (SOD, GPx, CAT, GST), liver histopathology and studies for the hepatic levels of NfkB, IL-6, TNF-α, MMP-3, MMP-12, Nrf2 and HO-1 were done. Significant (p≤0.05) toxicological alterations in the above parameters were recovered to normal in the treatment of NATQ combinations in SD rats. In conclusion, we provide evidence of protective efficacy of NATQ combination in alleviating the hepatotoxicities produced by CCLR in SD rats.
KW - Carbon tetrachloride
KW - MMP
KW - N-acetyl cysteine
KW - NfkB
KW - Radiation toxicity
KW - Thymoquinone
UR - https://www.scopus.com/pages/publications/85166954335
U2 - 10.5530/ijper.57.3.98
DO - 10.5530/ijper.57.3.98
M3 - Article
AN - SCOPUS:85166954335
SN - 0019-5464
VL - 57
SP - 802
EP - 812
JO - Indian Journal of Pharmaceutical Education and Research
JF - Indian Journal of Pharmaceutical Education and Research
IS - 3
ER -