Comparison of matched formalin-fixed paraffin embedded and fresh frozen meningioma tissue reveals bias in proteomic profiles

Hanan Schoffman, Yishai Levin, Ayelet Itzhaki-Alfia, Lea Tselekovits, Lior Gonen, Gilad Wolf Vainer, Goni Hout-Siloni, Iris Barshack, Zvi R. Cohen, Nevo Margalit, Tal Shahar

Research output: Contribution to journalArticlepeer-review

Abstract

Tissue biopsies are most commonly archived in a paraffin block following tissue fixation with formaldehyde (FFPE) or as fresh frozen tissue (FFT). While both methods preserve biological samples, little is known about how they affect the quantifiable proteome. We performed a ‘bottom-up’ proteomic analysis (N = 20) of short and long-term archived FFPE surgical samples of human meningiomas and compared them to matched FFT specimens. FFT facilitated a similar number of proteins assigned by MetaMorpheus compared with matched FFPE specimens (5378 vs. 5338 proteins, respectively (p = 0.053), regardless of archival time. However, marked differences in the proteome composition were apparent between FFPE and FFT specimens. Twenty-three percent of FFPE-derived peptides and 8% of FFT-derived peptides contained at least one chemical modification. Methylation and formylation were most prominent in FFPE-derived peptides (36% and 17% of modified FFPE peptides, respectively) while, most of phosphorylation and iron modifications appeared in FFT-derived peptides (p < 0.001). A mean 14% (± 2.9) of peptides identified in FFPE contained at least one modified Lysine residue. Importantly, larger proteins were significantly overrepresented in FFT specimens, while FFPE specimens were enriched with smaller proteins.

Original languageEnglish
Article number2200085
JournalProteomics
Volume22
Issue number21
DOIs
StatePublished - 1 Nov 2022
Externally publishedYes

Keywords

  • formalin-fixed paraffin-embedded tissue, mass spectrometry, meningioma, proteomics
  • fresh frozen tissue

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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