Comparison of stimulated tissue factor expression by brain microvascular endothelial cells from normotensive (WKY) and hypertensive (SHR) rats

David A. Doron; Richard M. McCarron; Eliahu Heldman; Anna Leena Sirén; Maria Spatz; Giora Feuerstein; Harvey B. Pollard; John M. Hallenbeck

doi:10.1016/0006-8993(92)91493-X

Comparison of stimulated tissue factor expression by brain microvascular endothelial cells from normotensive (WKY) and hypertensive (SHR) rats

David A. Doron, Richard M. McCarron, Eliahu Heldman, Anna Leena Sirén, Maria Spatz, Giora Feuerstein, Harvey B. Pollard, John M. Hallenbeck

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The amounts of tissue factor (TF) expressed by brain microvascular endothelial cells (BMECs) from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were compared after stimulating the cells with different doses of lipopolysaccharide (LPS), thrombin, phorbol myristic acid (PMA), Ca²⁺-ionophore (A23187), or tumor necrosis factor (TNF) and interleukin-1 (IL-1). Treatment of cultured BMECs from WKY and SHR with all of these factors dose-dependently increased their total amount of TF; no substantive differences in the levels of enhanced TF expression were observed between WKY and SHR BMECs. We conclude that stimulated endothelium from rats with hypertension, a major stroke risk factor, is not hyperresponsive with respect to TF expression when compared to normotensive controls.

Original language	English
Pages (from-to)	346-349
Number of pages	4
Journal	Brain Research
Volume	597
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(92)91493-X
State	Published - 4 Dec 1992
Externally published	Yes

Keywords

Endothelium
Lipopolysaccharide
Thromboplastin

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0006-8993(92)91493-X

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title = "Comparison of stimulated tissue factor expression by brain microvascular endothelial cells from normotensive (WKY) and hypertensive (SHR) rats",

abstract = "The amounts of tissue factor (TF) expressed by brain microvascular endothelial cells (BMECs) from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were compared after stimulating the cells with different doses of lipopolysaccharide (LPS), thrombin, phorbol myristic acid (PMA), Ca2+-ionophore (A23187), or tumor necrosis factor (TNF) and interleukin-1 (IL-1). Treatment of cultured BMECs from WKY and SHR with all of these factors dose-dependently increased their total amount of TF; no substantive differences in the levels of enhanced TF expression were observed between WKY and SHR BMECs. We conclude that stimulated endothelium from rats with hypertension, a major stroke risk factor, is not hyperresponsive with respect to TF expression when compared to normotensive controls.",

keywords = "Endothelium, Lipopolysaccharide, Thromboplastin",

author = "Doron, {David A.} and McCarron, {Richard M.} and Eliahu Heldman and Sir{\'e}n, {Anna Leena} and Maria Spatz and Giora Feuerstein and Pollard, {Harvey B.} and Hallenbeck, {John M.}",

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doi = "10.1016/0006-8993(92)91493-X",

language = "English",

volume = "597",

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T1 - Comparison of stimulated tissue factor expression by brain microvascular endothelial cells from normotensive (WKY) and hypertensive (SHR) rats

AU - Doron, David A.

AU - McCarron, Richard M.

AU - Heldman, Eliahu

AU - Sirén, Anna Leena

AU - Spatz, Maria

AU - Feuerstein, Giora

AU - Pollard, Harvey B.

AU - Hallenbeck, John M.

PY - 1992/12/4

Y1 - 1992/12/4

N2 - The amounts of tissue factor (TF) expressed by brain microvascular endothelial cells (BMECs) from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were compared after stimulating the cells with different doses of lipopolysaccharide (LPS), thrombin, phorbol myristic acid (PMA), Ca2+-ionophore (A23187), or tumor necrosis factor (TNF) and interleukin-1 (IL-1). Treatment of cultured BMECs from WKY and SHR with all of these factors dose-dependently increased their total amount of TF; no substantive differences in the levels of enhanced TF expression were observed between WKY and SHR BMECs. We conclude that stimulated endothelium from rats with hypertension, a major stroke risk factor, is not hyperresponsive with respect to TF expression when compared to normotensive controls.

AB - The amounts of tissue factor (TF) expressed by brain microvascular endothelial cells (BMECs) from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were compared after stimulating the cells with different doses of lipopolysaccharide (LPS), thrombin, phorbol myristic acid (PMA), Ca2+-ionophore (A23187), or tumor necrosis factor (TNF) and interleukin-1 (IL-1). Treatment of cultured BMECs from WKY and SHR with all of these factors dose-dependently increased their total amount of TF; no substantive differences in the levels of enhanced TF expression were observed between WKY and SHR BMECs. We conclude that stimulated endothelium from rats with hypertension, a major stroke risk factor, is not hyperresponsive with respect to TF expression when compared to normotensive controls.

KW - Endothelium

KW - Lipopolysaccharide

KW - Thromboplastin

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U2 - 10.1016/0006-8993(92)91493-X

DO - 10.1016/0006-8993(92)91493-X

M3 - Article

AN - SCOPUS:0026747245

SN - 0006-8993

VL - 597

SP - 346

EP - 349

JO - Brain Research

JF - Brain Research

IS - 2

ER -

Comparison of stimulated tissue factor expression by brain microvascular endothelial cells from normotensive (WKY) and hypertensive (SHR) rats

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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