Comparison of stimulated tissue factor expression by brain microvascular endothelial cells from normotensive (WKY) and hypertensive (SHR) rats

David A. Doron, Richard M. McCarron, Eliahu Heldman, Anna Leena Sirén, Maria Spatz, Giora Feuerstein, Harvey B. Pollard, John M. Hallenbeck

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The amounts of tissue factor (TF) expressed by brain microvascular endothelial cells (BMECs) from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were compared after stimulating the cells with different doses of lipopolysaccharide (LPS), thrombin, phorbol myristic acid (PMA), Ca2+-ionophore (A23187), or tumor necrosis factor (TNF) and interleukin-1 (IL-1). Treatment of cultured BMECs from WKY and SHR with all of these factors dose-dependently increased their total amount of TF; no substantive differences in the levels of enhanced TF expression were observed between WKY and SHR BMECs. We conclude that stimulated endothelium from rats with hypertension, a major stroke risk factor, is not hyperresponsive with respect to TF expression when compared to normotensive controls.

Original languageEnglish
Pages (from-to)346-349
Number of pages4
JournalBrain Research
Volume597
Issue number2
DOIs
StatePublished - 4 Dec 1992
Externally publishedYes

Keywords

  • Endothelium
  • Lipopolysaccharide
  • Thromboplastin

ASJC Scopus subject areas

  • Neuroscience (all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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