Computational characterization and protein network interactions of auxin efflux carrier proteins

Auxins are one of the major phytohormones found in plants, belongs to the transport membrane that plays a crucial role in the development and growth of plant by regulating various cellular processes from cell division to root organogenesis. In this study the computational approach was used to compute the various functional properties of auxin efflux carrier proteins from Arabidopsis lyrata, Arabidopsis thaliana, Glycine max, Oryza sativa sub specie indica, Populus trichocarpa and Zea mays. The functional characterization of the auxin efflux carrier was analyzed by SOSUI followed by physicochemical properties was analysed by using ProtParam. It was found that the isoelectric point (pI) was found to be ranged from 5.64 to 9.47 and instability index ranged 23.48 to 46.17. The aliphatic index ranged from 82.07 to 127.72 suggesting proteins are highly thermostable. The secondary Structure prediction suggested the dominance of random coils in comparison to alpha helix and beta sheets. To infer the interactions of auxin efflux carrier within organism, we used STRING V 10 database. The objective of this study is to develop a protein- protein interaction network based on functional, structural and phylogenetic analysis within Arabidopsis lyrata, Arabidopsis thaliana, Glycine max, Oryza sativa sub specie indica, Populustrichocarpa and Zea mays.