Construction and recovery of viable retroviral genomes carrying a bacterial suppressor transfer RNA gene

Leslie I. Lobel; Mayuri Patel; Walter King; M. Chi Nguyen-Huu; Stephen P. Goff

doi:10.1126/science.2984770

Construction and recovery of viable retroviral genomes carrying a bacterial suppressor transfer RNA gene

Leslie I. Lobel
, Mayuri Patel
, Walter King
, M. Chi Nguyen-Huu
, Stephen P. Goff

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The integration of retroviral genomes into cellular DNA can induce mutations by altering the expression of nearby cellular genes and can serve to identify the gene affected. The construction of a retrovirus that stably carries a suppressor transfer RNA gene from Escherichia coli has allowed facile recovery of the viral genome in vectors marked with amber mutations. This virus can be used for rapid isolation of cellular sequences at the site of proviral insertion.

Original language	English
Pages (from-to)	329-332
Number of pages	4
Journal	Science
Volume	228
Issue number	4697
DOIs	https://doi.org/10.1126/science.2984770
State	Published - 1 Jan 1985
Externally published	Yes

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title = "Construction and recovery of viable retroviral genomes carrying a bacterial suppressor transfer RNA gene",

abstract = "The integration of retroviral genomes into cellular DNA can induce mutations by altering the expression of nearby cellular genes and can serve to identify the gene affected. The construction of a retrovirus that stably carries a suppressor transfer RNA gene from Escherichia coli has allowed facile recovery of the viral genome in vectors marked with amber mutations. This virus can be used for rapid isolation of cellular sequences at the site of proviral insertion.",

author = "Lobel, \{Leslie I.\} and Mayuri Patel and Walter King and Nguyen-Huu, \{M. Chi\} and Goff, \{Stephen P.\}",

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doi = "10.1126/science.2984770",

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AU - Lobel, Leslie I.

AU - Patel, Mayuri

AU - King, Walter

AU - Nguyen-Huu, M. Chi

AU - Goff, Stephen P.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - The integration of retroviral genomes into cellular DNA can induce mutations by altering the expression of nearby cellular genes and can serve to identify the gene affected. The construction of a retrovirus that stably carries a suppressor transfer RNA gene from Escherichia coli has allowed facile recovery of the viral genome in vectors marked with amber mutations. This virus can be used for rapid isolation of cellular sequences at the site of proviral insertion.

AB - The integration of retroviral genomes into cellular DNA can induce mutations by altering the expression of nearby cellular genes and can serve to identify the gene affected. The construction of a retrovirus that stably carries a suppressor transfer RNA gene from Escherichia coli has allowed facile recovery of the viral genome in vectors marked with amber mutations. This virus can be used for rapid isolation of cellular sequences at the site of proviral insertion.

UR - https://www.scopus.com/pages/publications/0021884201

U2 - 10.1126/science.2984770

DO - 10.1126/science.2984770

M3 - Article

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AN - SCOPUS:0021884201

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SP - 329

EP - 332

JO - Science

JF - Science

IS - 4697

ER -

Construction and recovery of viable retroviral genomes carrying a bacterial suppressor transfer RNA gene

Abstract

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