Construction of P1-derived artificial chromosome and yeast artificial chromosome contigs encompassing the DFNB7 and DFNB11 region of chromosome 9q13-21

John H. Greinwald; Daryl A. Scott; Jacquie R. Marietta; Rivka Carmi; Jose Manaligod; Arabandi Ramesh; Ross I.S. Zbar; Michelle L. Kraft; Khalil Elbedour; Yael Yairi; Maurice Musy; Anne B. Skvorak; Guy Van Camp; C. R.Srikumari Srisailapathy; Michael Lovett; Cynthia C. Morton; Val C. Sheffield; Richard J.H. Smith

doi:10.1101/gr.7.9.879

Construction of P1-derived artificial chromosome and yeast artificial chromosome contigs encompassing the DFNB7 and DFNB11 region of chromosome 9q13-21

John H. Greinwald, Daryl A. Scott, Jacquie R. Marietta, Rivka Carmi, Jose Manaligod, Arabandi Ramesh, Ross I.S. Zbar, Michelle L. Kraft, Khalil Elbedour, Yael Yairi, Maurice Musy, Anne B. Skvorak, Guy Van Camp, C. R.Srikumari Srisailapathy, Michael Lovett, Cynthia C. Morton, Val C. Sheffield, Richard J.H. Smith

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

DFNB7 and DFNB11, two loci for autosomal recessive nonsyndromic hearing loss (ARNSHL), have been mapped to chromosome 9q13-21 in separate consanguineous families. Using a radiation hybrid map, we have determined the correct marker order in the DFNB7/11 region and have demonstrated that the DFNB11 locus resides within a redefined DFNB7 interval. The gene(s) responsible for ARNSHL at these loci resides within an ~ 1 cM interval bounded by markers DPS1806 (centromeric) and DPS769 (telomeric). A recently discovered Indian family confirms the new telomeric boundary. To assist in the identification and cloning of candidate genes, YAC and PAC contigs were constructed. A total of 19 YAC and 23 PAC clones were utilized to span the affected region and ensure double coverage throughout. Twenty-two previously published STSs and 21 new STSs were used to determine marker order and confirm the integrity of the contig. Using a positional cloning strategy we have identified three cochlear expressed genes that map to the DFNB7/11 interval.

Original language	English
Pages (from-to)	879-886
Number of pages	8
Journal	Genome Research
Volume	7
Issue number	9
DOIs	https://doi.org/10.1101/gr.7.9.879
State	Published - 1 Jan 1997

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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Greinwald, J. H., Scott, D. A., Marietta, J. R., Carmi, R., Manaligod, J., Ramesh, A., Zbar, R. I. S., Kraft, M. L., Elbedour, K., Yairi, Y., Musy, M., Skvorak, A. B., Van Camp, G., Srisailapathy, C. R. S., Lovett, M., Morton, C. C., Sheffield, V. C., & Smith, R. J. H. (1997). Construction of P1-derived artificial chromosome and yeast artificial chromosome contigs encompassing the DFNB7 and DFNB11 region of chromosome 9q13-21. Genome Research, 7(9), 879-886. https://doi.org/10.1101/gr.7.9.879

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title = "Construction of P1-derived artificial chromosome and yeast artificial chromosome contigs encompassing the DFNB7 and DFNB11 region of chromosome 9q13-21",

abstract = "DFNB7 and DFNB11, two loci for autosomal recessive nonsyndromic hearing loss (ARNSHL), have been mapped to chromosome 9q13-21 in separate consanguineous families. Using a radiation hybrid map, we have determined the correct marker order in the DFNB7/11 region and have demonstrated that the DFNB11 locus resides within a redefined DFNB7 interval. The gene(s) responsible for ARNSHL at these loci resides within an ~ 1 cM interval bounded by markers DPS1806 (centromeric) and DPS769 (telomeric). A recently discovered Indian family confirms the new telomeric boundary. To assist in the identification and cloning of candidate genes, YAC and PAC contigs were constructed. A total of 19 YAC and 23 PAC clones were utilized to span the affected region and ensure double coverage throughout. Twenty-two previously published STSs and 21 new STSs were used to determine marker order and confirm the integrity of the contig. Using a positional cloning strategy we have identified three cochlear expressed genes that map to the DFNB7/11 interval.",

author = "Greinwald, {John H.} and Scott, {Daryl A.} and Marietta, {Jacquie R.} and Rivka Carmi and Jose Manaligod and Arabandi Ramesh and Zbar, {Ross I.S.} and Kraft, {Michelle L.} and Khalil Elbedour and Yael Yairi and Maurice Musy and Skvorak, {Anne B.} and {Van Camp}, Guy and Srisailapathy, {C. R.Srikumari} and Michael Lovett and Morton, {Cynthia C.} and Sheffield, {Val C.} and Smith, {Richard J.H.}",

year = "1997",

month = jan,

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doi = "10.1101/gr.7.9.879",

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Greinwald, JH, Scott, DA, Marietta, JR, Carmi, R, Manaligod, J, Ramesh, A, Zbar, RIS, Kraft, ML, Elbedour, K, Yairi, Y, Musy, M, Skvorak, AB, Van Camp, G, Srisailapathy, CRS, Lovett, M, Morton, CC, Sheffield, VC & Smith, RJH 1997, 'Construction of P1-derived artificial chromosome and yeast artificial chromosome contigs encompassing the DFNB7 and DFNB11 region of chromosome 9q13-21', Genome Research, vol. 7, no. 9, pp. 879-886. https://doi.org/10.1101/gr.7.9.879

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T1 - Construction of P1-derived artificial chromosome and yeast artificial chromosome contigs encompassing the DFNB7 and DFNB11 region of chromosome 9q13-21

AU - Greinwald, John H.

AU - Scott, Daryl A.

AU - Marietta, Jacquie R.

AU - Carmi, Rivka

AU - Manaligod, Jose

AU - Ramesh, Arabandi

AU - Zbar, Ross I.S.

AU - Kraft, Michelle L.

AU - Elbedour, Khalil

AU - Yairi, Yael

AU - Musy, Maurice

AU - Skvorak, Anne B.

AU - Van Camp, Guy

AU - Srisailapathy, C. R.Srikumari

AU - Lovett, Michael

AU - Morton, Cynthia C.

AU - Sheffield, Val C.

AU - Smith, Richard J.H.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - DFNB7 and DFNB11, two loci for autosomal recessive nonsyndromic hearing loss (ARNSHL), have been mapped to chromosome 9q13-21 in separate consanguineous families. Using a radiation hybrid map, we have determined the correct marker order in the DFNB7/11 region and have demonstrated that the DFNB11 locus resides within a redefined DFNB7 interval. The gene(s) responsible for ARNSHL at these loci resides within an ~ 1 cM interval bounded by markers DPS1806 (centromeric) and DPS769 (telomeric). A recently discovered Indian family confirms the new telomeric boundary. To assist in the identification and cloning of candidate genes, YAC and PAC contigs were constructed. A total of 19 YAC and 23 PAC clones were utilized to span the affected region and ensure double coverage throughout. Twenty-two previously published STSs and 21 new STSs were used to determine marker order and confirm the integrity of the contig. Using a positional cloning strategy we have identified three cochlear expressed genes that map to the DFNB7/11 interval.

AB - DFNB7 and DFNB11, two loci for autosomal recessive nonsyndromic hearing loss (ARNSHL), have been mapped to chromosome 9q13-21 in separate consanguineous families. Using a radiation hybrid map, we have determined the correct marker order in the DFNB7/11 region and have demonstrated that the DFNB11 locus resides within a redefined DFNB7 interval. The gene(s) responsible for ARNSHL at these loci resides within an ~ 1 cM interval bounded by markers DPS1806 (centromeric) and DPS769 (telomeric). A recently discovered Indian family confirms the new telomeric boundary. To assist in the identification and cloning of candidate genes, YAC and PAC contigs were constructed. A total of 19 YAC and 23 PAC clones were utilized to span the affected region and ensure double coverage throughout. Twenty-two previously published STSs and 21 new STSs were used to determine marker order and confirm the integrity of the contig. Using a positional cloning strategy we have identified three cochlear expressed genes that map to the DFNB7/11 interval.

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U2 - 10.1101/gr.7.9.879

DO - 10.1101/gr.7.9.879

M3 - Article

C2 - 9314493

AN - SCOPUS:0030839583

SN - 1088-9051

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SP - 879

EP - 886

JO - Genome Research

JF - Genome Research

IS - 9

ER -

Construction of P1-derived artificial chromosome and yeast artificial chromosome contigs encompassing the DFNB7 and DFNB11 region of chromosome 9q13-21

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