TY - JOUR
T1 - Contacts among non-sister dendritic branches at bifurcations shape neighboring dendrites and pattern their synaptic inputs
AU - Cove, Joshua
AU - Blinder, Pablo
AU - Baranes, Danny
N1 - Funding Information:
We would like to thank Drs. Elia Abi Jaoude and Myriam Lafrenier-Roula for performing the initial experiments. This project was supported by the Canada Foundation of Innovation, and partially by the Horowitz foundation.
PY - 2009/1/28
Y1 - 2009/1/28
N2 - The size and shape of neuronal dendritic arbors affect the number and pattern of synaptic inputs, as well as the complexity and function of brain circuits. However, the means by which different dendritic arbors take their final shape and how these shapes are associated with distinct synaptic patterns is still largely unknown. Dendritic ramification is influenced by dendrite-dendrite interactions that stabilize specific branching directions and ensure appropriate synaptic contacts. Yet, it is not clear by which mechanism these contacts are allocated. We found that stable dendro-dendritic contacts occur preferentially between non-sister dendritic branches at sites of bifurcations, and that this process is promoted by synaptic activity. Moreover, these contacts are associated with synaptic connections of higher density, higher level of synaptophysin, NR1, GluR2 subunits of glutamate receptors and elevated secretion capability than synaptic connections found on contacts made by non-bifurcating branches or along non-contacting parts of the dendrites. Thus, in cultured neurons, stabilization of hetero-neuronal dendro-dendritic contacts at bifurcations is a new mean to pattern and associate morphogenesis and synaptic input distribution in neighboring dendritic trees.
AB - The size and shape of neuronal dendritic arbors affect the number and pattern of synaptic inputs, as well as the complexity and function of brain circuits. However, the means by which different dendritic arbors take their final shape and how these shapes are associated with distinct synaptic patterns is still largely unknown. Dendritic ramification is influenced by dendrite-dendrite interactions that stabilize specific branching directions and ensure appropriate synaptic contacts. Yet, it is not clear by which mechanism these contacts are allocated. We found that stable dendro-dendritic contacts occur preferentially between non-sister dendritic branches at sites of bifurcations, and that this process is promoted by synaptic activity. Moreover, these contacts are associated with synaptic connections of higher density, higher level of synaptophysin, NR1, GluR2 subunits of glutamate receptors and elevated secretion capability than synaptic connections found on contacts made by non-bifurcating branches or along non-contacting parts of the dendrites. Thus, in cultured neurons, stabilization of hetero-neuronal dendro-dendritic contacts at bifurcations is a new mean to pattern and associate morphogenesis and synaptic input distribution in neighboring dendritic trees.
KW - Dendrite morphology
KW - Dendro-dendritic contact
KW - Synaptic connection
KW - Synaptic distribution
KW - Synaptic strength
UR - http://www.scopus.com/inward/record.url?scp=58149382410&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2008.11.028
DO - 10.1016/j.brainres.2008.11.028
M3 - Article
AN - SCOPUS:58149382410
SN - 0006-8993
VL - 1251
SP - 30
EP - 41
JO - Brain Research
JF - Brain Research
ER -