Continuous perfusion of mammalian cells embedded in agarose gel threads

David L. Foxall; Jack S. Cohen; James B. Mitchell

doi:10.1016/0014-4827(84)90176-9

Continuous perfusion of mammalian cells embedded in agarose gel threads

David L. Foxall, Jack S. Cohen, James B. Mitchell

Research output: Contribution to journal › Article › peer-review

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Abstract

A method for perfusing cells by embedding them in fine agarose gel threads is described and characterized. The rate of diffusion of a metabolite into the gel threads is determined by ³¹P-NMR spectroscopy. This perfusion method is shown to enable Chinese hamster lung fibroblasts (CHLF) to remain in a metabolically active state with high levels of intracellular ATP for many hours.

Original language	English
Pages (from-to)	521-529
Number of pages	9
Journal	Experimental Cell Research
Volume	154
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(84)90176-9
State	Published - 1 Jan 1984
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

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10.1016/0014-4827(84)90176-9

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title = "Continuous perfusion of mammalian cells embedded in agarose gel threads",

abstract = "A method for perfusing cells by embedding them in fine agarose gel threads is described and characterized. The rate of diffusion of a metabolite into the gel threads is determined by 31P-NMR spectroscopy. This perfusion method is shown to enable Chinese hamster lung fibroblasts (CHLF) to remain in a metabolically active state with high levels of intracellular ATP for many hours.",

author = "Foxall, {David L.} and Cohen, {Jack S.} and Mitchell, {James B.}",

year = "1984",

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doi = "10.1016/0014-4827(84)90176-9",

language = "English",

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pages = "521--529",

journal = "Experimental Cell Research",

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T1 - Continuous perfusion of mammalian cells embedded in agarose gel threads

AU - Foxall, David L.

AU - Cohen, Jack S.

AU - Mitchell, James B.

PY - 1984/1/1

Y1 - 1984/1/1

N2 - A method for perfusing cells by embedding them in fine agarose gel threads is described and characterized. The rate of diffusion of a metabolite into the gel threads is determined by 31P-NMR spectroscopy. This perfusion method is shown to enable Chinese hamster lung fibroblasts (CHLF) to remain in a metabolically active state with high levels of intracellular ATP for many hours.

AB - A method for perfusing cells by embedding them in fine agarose gel threads is described and characterized. The rate of diffusion of a metabolite into the gel threads is determined by 31P-NMR spectroscopy. This perfusion method is shown to enable Chinese hamster lung fibroblasts (CHLF) to remain in a metabolically active state with high levels of intracellular ATP for many hours.

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U2 - 10.1016/0014-4827(84)90176-9

DO - 10.1016/0014-4827(84)90176-9

M3 - Article

C2 - 6479241

AN - SCOPUS:0021149275

SN - 0014-4827

VL - 154

SP - 521

EP - 529

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Continuous perfusion of mammalian cells embedded in agarose gel threads

Abstract

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