TY - JOUR
T1 - Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium
AU - Mytlis, Avishag
AU - Kumar, Vineet
AU - Qiu, Tao
AU - Deis, Rachael
AU - Hart, Neta
AU - Levy, Karine
AU - Masek, Markus
AU - Shawahny, Amal
AU - Ahmad, Adam
AU - Eitan, Hagai
AU - Nather, Farouq
AU - Adar-Levor, Shai
AU - Birnbaum, Ramon Y.
AU - Elia, Natalie
AU - Bachmann-Gagescu, Ruxandra
AU - Roy, Sudipto
AU - Elkouby, Yaniv M.
N1 - Publisher Copyright:
© 2022 American Association for the Advancement of Science. All rights reserved.
PY - 2022/6/17
Y1 - 2022/6/17
N2 - A hallmark of meiosis is chromosomal pairing, which requires telomere tethering and rotation on the nuclear envelope through microtubules, driving chromosome homology searches. Telomere pulling toward the centrosome forms the "zygotene chromosomal bouquet." Here, we identified the "zygotene cilium" in oocytes. This cilium provides a cable system for the bouquet machinery and extends throughout the germline cyst. Using zebrafish mutants and live manipulations, we demonstrate that the cilium anchors the centrosome to counterbalance telomere pulling. The cilium is essential for bouquet and synaptonemal complex formation, oogenesis, ovarian development, and fertility. Thus, a cilium represents a conserved player in zebrafish and mouse meiosis, which sheds light on reproductive aspects in ciliopathies and suggests that cilia can control chromosomal dynamics.
AB - A hallmark of meiosis is chromosomal pairing, which requires telomere tethering and rotation on the nuclear envelope through microtubules, driving chromosome homology searches. Telomere pulling toward the centrosome forms the "zygotene chromosomal bouquet." Here, we identified the "zygotene cilium" in oocytes. This cilium provides a cable system for the bouquet machinery and extends throughout the germline cyst. Using zebrafish mutants and live manipulations, we demonstrate that the cilium anchors the centrosome to counterbalance telomere pulling. The cilium is essential for bouquet and synaptonemal complex formation, oogenesis, ovarian development, and fertility. Thus, a cilium represents a conserved player in zebrafish and mouse meiosis, which sheds light on reproductive aspects in ciliopathies and suggests that cilia can control chromosomal dynamics.
UR - http://www.scopus.com/inward/record.url?scp=85132452651&partnerID=8YFLogxK
U2 - 10.1126/science.abh3104
DO - 10.1126/science.abh3104
M3 - Article
C2 - 35549308
AN - SCOPUS:85132452651
SN - 0036-8075
VL - 376
JO - Science
JF - Science
IS - 6599
M1 - eabh3104
ER -