Controlling confinement and topology to study collective cell behaviors

Guillaume Duclos; Maxime Deforet; Hannah G. Yevick; Olivier Cochet-Escartin; Flora Ascione; Sarah Moitrier; Trinish Sarkar; Victor Yashunsky; Isabelle Bonnet; Axel Buguin; Pascal Silberzan

doi:10.1007/978-1-4939-7701-7_28

Controlling confinement and topology to study collective cell behaviors

Guillaume Duclos, Maxime Deforet, Hannah G. Yevick, Olivier Cochet-Escartin, Flora Ascione, Sarah Moitrier, Trinish Sarkar, Victor Yashunsky, Isabelle Bonnet, Axel Buguin, Pascal Silberzan

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

6 Scopus citations

Abstract

Confinement and substrate topology strongly affect the behavior of cell populations and, in particular, their collective migration. In vitro experiments dealing with these aspects require strategies of surface patterning that remain effective over long times (typically several days) and ways to control the surface topology in three dimensions. Here, we describe protocols addressing these two aspects. High-resolution patterning of a robust cell-repellent coating is achieved by etching the coating through a photoresist mask patterned directly on the coated surface. Out-of-plane curvature can be controlled using glass wires or corrugated “wavy” surfaces.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	387-399
Number of pages	13
DOIs	https://doi.org/10.1007/978-1-4939-7701-7_28
State	Published - 1 Jan 2018
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1749
ISSN (Print)	1064-3745

Keywords

Collective cell migration
Microfabrication
Negative curvature
Out-of-plane curvature
Positive curvature
Surface patterning

ASJC Scopus subject areas

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-7701-7_28

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Duclos, G., Deforet, M., Yevick, H. G., Cochet-Escartin, O., Ascione, F., Moitrier, S., Sarkar, T., Yashunsky, V., Bonnet, I., Buguin, A., & Silberzan, P. (2018). Controlling confinement and topology to study collective cell behaviors. In Methods in Molecular Biology (pp. 387-399). (Methods in Molecular Biology; Vol. 1749). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7701-7_28

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title = "Controlling confinement and topology to study collective cell behaviors",

abstract = "Confinement and substrate topology strongly affect the behavior of cell populations and, in particular, their collective migration. In vitro experiments dealing with these aspects require strategies of surface patterning that remain effective over long times (typically several days) and ways to control the surface topology in three dimensions. Here, we describe protocols addressing these two aspects. High-resolution patterning of a robust cell-repellent coating is achieved by etching the coating through a photoresist mask patterned directly on the coated surface. Out-of-plane curvature can be controlled using glass wires or corrugated “wavy” surfaces.",

keywords = "Collective cell migration, Microfabrication, Negative curvature, Out-of-plane curvature, Positive curvature, Surface patterning",

author = "Guillaume Duclos and Maxime Deforet and Yevick, {Hannah G.} and Olivier Cochet-Escartin and Flora Ascione and Sarah Moitrier and Trinish Sarkar and Victor Yashunsky and Isabelle Bonnet and Axel Buguin and Pascal Silberzan",

note = "Funding Information: We gratefully acknowledge financial support from the Groupement des Entreprises Fran{\c c}aises dans la Lutte contre le Cancer (GEFLUC) Ile-de-France, the R{\'e}gion Ile-de-France Domaine d{\textquoteright}Int{\'e}r{\^e}t Majeur (DIM) Nano-K, the Association pour la Recherche sur le Cancer (ARC), the EU cofund PRESTIGE post-doc program, the EU cofund IC-3i PhD program, and the Fondation Pierre-Gilles de Gennes. The “Biology-inspired Physics at MesoScales” group is a member of the CelTisPhyBio Labex and of the Institut Pierre-Gilles de Gennes. It is a pleasure to thank Mohamed El Beheiry for his help in the 3D processing of our images. Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC.",

year = "2018",

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doi = "10.1007/978-1-4939-7701-7_28",

language = "English",

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Duclos, G, Deforet, M, Yevick, HG, Cochet-Escartin, O, Ascione, F, Moitrier, S, Sarkar, T, Yashunsky, V, Bonnet, I, Buguin, A & Silberzan, P 2018, Controlling confinement and topology to study collective cell behaviors. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1749, Humana Press Inc., pp. 387-399. https://doi.org/10.1007/978-1-4939-7701-7_28

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T1 - Controlling confinement and topology to study collective cell behaviors

AU - Duclos, Guillaume

AU - Deforet, Maxime

AU - Yevick, Hannah G.

AU - Cochet-Escartin, Olivier

AU - Ascione, Flora

AU - Moitrier, Sarah

AU - Sarkar, Trinish

AU - Yashunsky, Victor

AU - Bonnet, Isabelle

AU - Buguin, Axel

AU - Silberzan, Pascal

N1 - Funding Information: We gratefully acknowledge financial support from the Groupement des Entreprises Françaises dans la Lutte contre le Cancer (GEFLUC) Ile-de-France, the Région Ile-de-France Domaine d’Intérêt Majeur (DIM) Nano-K, the Association pour la Recherche sur le Cancer (ARC), the EU cofund PRESTIGE post-doc program, the EU cofund IC-3i PhD program, and the Fondation Pierre-Gilles de Gennes. The “Biology-inspired Physics at MesoScales” group is a member of the CelTisPhyBio Labex and of the Institut Pierre-Gilles de Gennes. It is a pleasure to thank Mohamed El Beheiry for his help in the 3D processing of our images. Publisher Copyright: © 2018, Springer Science+Business Media, LLC.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Confinement and substrate topology strongly affect the behavior of cell populations and, in particular, their collective migration. In vitro experiments dealing with these aspects require strategies of surface patterning that remain effective over long times (typically several days) and ways to control the surface topology in three dimensions. Here, we describe protocols addressing these two aspects. High-resolution patterning of a robust cell-repellent coating is achieved by etching the coating through a photoresist mask patterned directly on the coated surface. Out-of-plane curvature can be controlled using glass wires or corrugated “wavy” surfaces.

AB - Confinement and substrate topology strongly affect the behavior of cell populations and, in particular, their collective migration. In vitro experiments dealing with these aspects require strategies of surface patterning that remain effective over long times (typically several days) and ways to control the surface topology in three dimensions. Here, we describe protocols addressing these two aspects. High-resolution patterning of a robust cell-repellent coating is achieved by etching the coating through a photoresist mask patterned directly on the coated surface. Out-of-plane curvature can be controlled using glass wires or corrugated “wavy” surfaces.

KW - Collective cell migration

KW - Microfabrication

KW - Negative curvature

KW - Out-of-plane curvature

KW - Positive curvature

KW - Surface patterning

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DO - 10.1007/978-1-4939-7701-7_28

M3 - Chapter

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AN - SCOPUS:85043778208

T3 - Methods in Molecular Biology

SP - 387

EP - 399

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Controlling confinement and topology to study collective cell behaviors

Abstract

Publication series

Keywords

ASJC Scopus subject areas

UN SDGs

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