Coordinate induction of glutathione biosynthesis and glutathione- metabolizing enzymes is correlated with salt tolerance in tomato

Valentina Mittova; Frederica L. Theodoulou; Guy Kiddle; Leonardo Gómez; Micha Volokita; Moshe Tal; Christine H. Foyer; Micha Guy

doi:10.1016/S0014-5793(03)01214-6

Coordinate induction of glutathione biosynthesis and glutathione- metabolizing enzymes is correlated with salt tolerance in tomato

Valentina Mittova, Frederica L. Theodoulou, Guy Kiddle, Leonardo Gómez, Micha Volokita, Moshe Tal, Christine H. Foyer, Micha Guy

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

134 Scopus citations

Abstract

The acclimation of reduced glutathione (GSH) biosynthesis and GSH-utilizing enzymes to salt stress was studied in two tomato species that differ in stress tolerance. Salt increased GSH content and GSH:GSSG (oxidized glutathione) ratio in oxidative stress-tolerant Lycopersicon pennellii (Lpa) but not in Lycopersicon esculentum (Lem). These changes were associated with salt-induced upregulation of γ-glutamylcysteine synthetase protein, an effect which was prevented by preincubation with buthionine sulfoximine. Salt treatment induced glutathione peroxidase and glutathione-S-transferase but not glutathione reductase activities in Lpa. These results suggest a mechanism of coordinate upregulation of synthesis and metabolism of GSH in Lpa, that is absent from Lem.

Original language	English
Pages (from-to)	417-421
Number of pages	5
Journal	FEBS Letters
Volume	554
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(03)01214-6
State	Published - 20 Nov 2003

Keywords

Glutathione
Glutathione peroxidase
Glutathione-S-transferase
Salt tolerance
Tomato
γ-Glutamylcysteine synthetase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/S0014-5793(03)01214-6

Cite this

@article{a05d654d8a5f4f5cb045f0d101886b9d,

title = "Coordinate induction of glutathione biosynthesis and glutathione- metabolizing enzymes is correlated with salt tolerance in tomato",

abstract = "The acclimation of reduced glutathione (GSH) biosynthesis and GSH-utilizing enzymes to salt stress was studied in two tomato species that differ in stress tolerance. Salt increased GSH content and GSH:GSSG (oxidized glutathione) ratio in oxidative stress-tolerant Lycopersicon pennellii (Lpa) but not in Lycopersicon esculentum (Lem). These changes were associated with salt-induced upregulation of γ-glutamylcysteine synthetase protein, an effect which was prevented by preincubation with buthionine sulfoximine. Salt treatment induced glutathione peroxidase and glutathione-S-transferase but not glutathione reductase activities in Lpa. These results suggest a mechanism of coordinate upregulation of synthesis and metabolism of GSH in Lpa, that is absent from Lem.",

keywords = "Glutathione, Glutathione peroxidase, Glutathione-S-transferase, Salt tolerance, Tomato, γ-Glutamylcysteine synthetase",

author = "Valentina Mittova and Theodoulou, {Frederica L.} and Guy Kiddle and Leonardo G{\'o}mez and Micha Volokita and Moshe Tal and Foyer, {Christine H.} and Micha Guy",

note = "Funding Information: The study was supported in part by the Dr. Herman Kessel Research Fund, in the memory of Mr. C.J.J. Van Kensbury. V.M. is a Jacob Blaustein fellowship incumbent. We are grateful to Dr. Frank Van Breusegem for the generous gift of plasmid mGR/pBluescript SKII−. Rothamsted Research receives grant-aided support from the BBSRC of the UK. ",

year = "2003",

month = nov,

day = "20",

doi = "10.1016/S0014-5793(03)01214-6",

language = "English",

volume = "554",

pages = "417--421",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "3",

}

TY - JOUR

T1 - Coordinate induction of glutathione biosynthesis and glutathione- metabolizing enzymes is correlated with salt tolerance in tomato

AU - Mittova, Valentina

AU - Theodoulou, Frederica L.

AU - Kiddle, Guy

AU - Gómez, Leonardo

AU - Volokita, Micha

AU - Tal, Moshe

AU - Foyer, Christine H.

AU - Guy, Micha

N1 - Funding Information: The study was supported in part by the Dr. Herman Kessel Research Fund, in the memory of Mr. C.J.J. Van Kensbury. V.M. is a Jacob Blaustein fellowship incumbent. We are grateful to Dr. Frank Van Breusegem for the generous gift of plasmid mGR/pBluescript SKII−. Rothamsted Research receives grant-aided support from the BBSRC of the UK.

PY - 2003/11/20

Y1 - 2003/11/20

N2 - The acclimation of reduced glutathione (GSH) biosynthesis and GSH-utilizing enzymes to salt stress was studied in two tomato species that differ in stress tolerance. Salt increased GSH content and GSH:GSSG (oxidized glutathione) ratio in oxidative stress-tolerant Lycopersicon pennellii (Lpa) but not in Lycopersicon esculentum (Lem). These changes were associated with salt-induced upregulation of γ-glutamylcysteine synthetase protein, an effect which was prevented by preincubation with buthionine sulfoximine. Salt treatment induced glutathione peroxidase and glutathione-S-transferase but not glutathione reductase activities in Lpa. These results suggest a mechanism of coordinate upregulation of synthesis and metabolism of GSH in Lpa, that is absent from Lem.

AB - The acclimation of reduced glutathione (GSH) biosynthesis and GSH-utilizing enzymes to salt stress was studied in two tomato species that differ in stress tolerance. Salt increased GSH content and GSH:GSSG (oxidized glutathione) ratio in oxidative stress-tolerant Lycopersicon pennellii (Lpa) but not in Lycopersicon esculentum (Lem). These changes were associated with salt-induced upregulation of γ-glutamylcysteine synthetase protein, an effect which was prevented by preincubation with buthionine sulfoximine. Salt treatment induced glutathione peroxidase and glutathione-S-transferase but not glutathione reductase activities in Lpa. These results suggest a mechanism of coordinate upregulation of synthesis and metabolism of GSH in Lpa, that is absent from Lem.

KW - Glutathione

KW - Glutathione peroxidase

KW - Glutathione-S-transferase

KW - Salt tolerance

KW - Tomato

KW - γ-Glutamylcysteine synthetase

UR - http://www.scopus.com/inward/record.url?scp=0242458201&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(03)01214-6

DO - 10.1016/S0014-5793(03)01214-6

M3 - Article

AN - SCOPUS:0242458201

SN - 0014-5793

VL - 554

SP - 417

EP - 421

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Coordinate induction of glutathione biosynthesis and glutathione- metabolizing enzymes is correlated with salt tolerance in tomato

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this