Cop9 signalosome subunit 8 (CSN8) is essential for Drosophila development

Pazit Oren-Giladi, Ofra Krieger, Bruce A. Edgar, Daniel A. Chamovitz, Daniel Segal

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The COP9 signalosome (CSN) is a multisubunit regulator highly conserved in evolution. We show here that CSN subunit 8 (CSN8) is essential for Drosophila development. CSN8 is maternally contributed and present throughout development. Null mutants generated in this study are larval lethal, showing phenotypes associated with mutations in either CSN4 (molting defects) or CSN5 (melanotic tumors). Analysis of mitotic and germ-line csn8null clones revealed the requirement of CSN8 for multiple developmental processes. The germ-line clones arrested at mid-oogenesis, while the mitotic clones led to deformed adult eyes or wings. CSN8 is present exclusively as part of the CSN holo-complex, and lack of CSN8 in the mutants leads to CSN instability. Consistent with this, Cullin deneddylation is impaired in the csn8null mutants.

Original languageEnglish
Pages (from-to)221-231
JournalGenes to Cells
Volume13
Issue number3
DOIs
StatePublished - 1 Mar 2008
Externally publishedYes

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