TY - JOUR
T1 - Coral-associated bacterial extracts inhibit cellular NF-κB pathway
AU - Oliver, Edwin
AU - Nandakumar, Natarajan
AU - Faibish, Hanny
AU - Gopas, Jacob
AU - Kushmaro, Ariel
N1 - Funding Information:
This work was supported ISF Grant 1169/07, The Israel Cancer Association through the Miriam and Shlomo Hasid Memorial fund, The Genetics and Cancer Goldyne Savad Estate, Ben Gurion University and The Richard H. Holzer Foundation. Acknowledgments
Funding Information:
The PBC research fellowship extended by the Planning and Budgeting Committee (PBC) of the Council for Higher Education in Israel, to E.O and NN is gratefully acknowledged. We gratefully acknowledge Hila Ben Hamo and Shimrit Laor for their help in collecting the samples from Red sea.
Publisher Copyright:
© 2017 The Author(s). This open access article is distributed under a Creative Commons Attribution (CC-BY) 4.0 license.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Marine natural products have recently been recognized as a promising source of NF-κB inhibitors. In this study, extracts of bacterial isolates from the coral Favis sp. were screened for NF-κB modulatory capability using an NF-κB luciferase reporter gene assay. The bacterial extracts had variable effects on NF-κB activity: from the 39 extracts tested, only one exhibited significant NF-κB inhibition and two caused NF-κB up regulation. In addition, we showed that coral bacteria New-33 extract inhibits NF-κB alternative pathway subunits in a non-cytotoxic manner. HPLC analysis confirmed the presence of a low molecular mass compound and NCBI megaBLAST revealed that the New-33 16S rRNA gene sequences are similar to Vibrio mediterranei. This specific modulation of the NF-κB by alternative pathway could contribute to therapeutic implications and also raises questions about host-symbiont interaction in the marine environment.
AB - Marine natural products have recently been recognized as a promising source of NF-κB inhibitors. In this study, extracts of bacterial isolates from the coral Favis sp. were screened for NF-κB modulatory capability using an NF-κB luciferase reporter gene assay. The bacterial extracts had variable effects on NF-κB activity: from the 39 extracts tested, only one exhibited significant NF-κB inhibition and two caused NF-κB up regulation. In addition, we showed that coral bacteria New-33 extract inhibits NF-κB alternative pathway subunits in a non-cytotoxic manner. HPLC analysis confirmed the presence of a low molecular mass compound and NCBI megaBLAST revealed that the New-33 16S rRNA gene sequences are similar to Vibrio mediterranei. This specific modulation of the NF-κB by alternative pathway could contribute to therapeutic implications and also raises questions about host-symbiont interaction in the marine environment.
KW - NF-κB
KW - Vibrio mediterranei
KW - host–symbiont interaction
KW - marine natural products
UR - http://www.scopus.com/inward/record.url?scp=85051779199&partnerID=8YFLogxK
U2 - 10.1080/23311843.2017.1292865
DO - 10.1080/23311843.2017.1292865
M3 - Article
AN - SCOPUS:85051779199
SN - 2331-1843
VL - 3
JO - Cogent Environmental Science
JF - Cogent Environmental Science
IS - 1
M1 - 1292865
ER -
