Tissue or explant culture is broadly defined as the in vitro maintenance of tissues and organs including their various components and provides information pertinent to the whole organism, by taking into account integrated physiological processes. Taking advantage of the innate ability of corals to quickly regenerate, explanted coral tissues are able to be micropropagated for long-term maintenance in vitro, for a variety of uses. The procedure described here provides a general method for the explantation of coral tissues, their micropropagation and maintenance. Due to innate differences between species, each requires culture optimization that takes into account specific physicochemical requirements for their cultivation. Tissue culture of coral is a viable technology that provides lines of genetically identical micropropagates, with or without skeleton and is amenable to controlled laboratory studies. This technology significantly enhances and accelerates studies of coral physiology, pathology and toxicology.
ASJC Scopus subject areas
- General Veterinary