Correlative Light Electron Microscopy as a Navigating Tool for Cryo-Electron Tomography Analysis

Melanie Bokstad, Ohad Medalia

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations


Modern microscopy techniques, including fluorescence and electron microscopy, have allowed us to gain insights into the molecular organization of cells. Despite the different views attained by fluorescent and electron microscopy, these approaches complement each other in visualizing multicomponent complexes in their native environment. Combining the strengths of both techniques could provide a more realistic view of cellular processes. During the last decade, cryo-electron tomography (cryo-ET) has become central to exploring cellular structures on a nanometric scale. By combining cryo-ET with fluorescence microscopy, it should be possible to localize proteins inside a cell with high precision and by these means to elucidate not only the macromolecular architecture but also the remodeling events during the many cellular processes. Thus, cryo-ET might have the potential to close the resolution gap between cellular and structural biology.

Original languageEnglish
Title of host publicationFluorescence Microscopy
Subtitle of host publicationSuper-Resolution and other Novel Techniques
PublisherElsevier Inc.
Number of pages11
ISBN (Electronic)9780124167131
ISBN (Print)9780124095137
StatePublished - 18 Mar 2014
Externally publishedYes


  • Cellular tomography
  • Correlative fluoresce and electron tomography
  • Cryo-electron tomography
  • Sample preparation for electron microscopy

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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