Corrigendum to: Application of stable-isotope labelling techniques for the detection of active diazotrophs (Environmental Microbiology, (2018), 20, 1, (44-61), 10.1111/1462-2920.13954)

In the above article, it came to the authors' attention that the ¹⁵N-N₂ gas used only for the ‘Comparison of ¹⁵N₂-tracer assay with the acetylene reduction assay’ (data in Figure 1A,C) contained traces of ¹⁵N-labeled ammonia gas (155.16 ppm). The authors repeated the experiment with ¹⁵N-N₂ gas free of such contamination and observed the same patterns, as shown in the figure below (Figure 1A,C); they detected incorporation of ¹⁵N with the ¹⁵N₂-tracer assay when nitrogenase activity was not detectable with the acetylene reduction assay. 1 FIGURE (Figure presented.) Comparison of ARA and ¹⁵N₂ tracer assay. ARA performed on a forest soil sample incubated with either fructose or artificial root exudates (RE) (panels A). ‘Soil, C₂H₄ contr.’ indicates an ethylene consumption control performed on the forest soil sample. ‘Soil, C₂H₂’ refers to soil samples incubated with acetylene in the ARA. Graphs depict average ethylene concentration (ppm) +/− standard error. Panels C depict the incorporation of ¹⁵N from ¹⁵N₂ gas (average δ¹⁵N +/− standard error) in the same soil samples used for ARA. ‘Contr.’ indicates incubation with lab air. Thus, this repeat confirmed that the application of the ¹⁵N₂-tracer assay had a higher sensitivity than the acetylene reduction assay. The updated results do not change the implications or interpretations of the study. The authors thank Gaute Lavik from the Max Planck Institute for Marine Microbiology, Bremen, Germany, for analyzing the ¹⁵N-N₂ gas samples.