CRISPR based bacterial genome editing and removal of pathogens

Ravi Jothi, Chandrasekar Karthika, Arumugam Kamaladevi, Lakkakula Satish, Shunmugiah Karutha Pandian, Shanmugaraj Gowrishankar

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

Engineering nucleases to achieve targeted genome editing has turned out to be a revolutionary means for manipulating the genetic content in diversified living organisms. For targeted genome editing, till to date, only three engineered nucleases exist viz. zinc finger nucleases, transcription activator–like effector nucleases and RNA-mediated nucleases (RGNs) (Cas nucleases) from the clustered regularly interspaced short palindromic repeat (CRISPR). Among, Cas9 nuclease has been considered as a simplest tool for efficient modification of endogenous genes in an extensive stretch of organisms, owing to its amenability to design guide RNA compatible to the sequence of new targets. Moreover, CRISPR/Cas system delivers a multipurpose RNA-guided DNA-targeting platform called as CRISPR interference (CRISPRi), as well as epigenetic modifications and high throughput screening in diverse organism including bacteria, all in a sequence explicit way. With these entire advancements, the present chapter illustrates the deployment of CRISPR/Cas9 in bacterial genome editing and removal of pathogens.

Original languageEnglish
Title of host publicationReprogramming the Genome
Subtitle of host publicationApplications of CRISPR-Cas in Non-mammalian Systems Part A
EditorsVijai Singh
PublisherElsevier B.V.
Pages77-92
Number of pages16
ISBN (Print)9780323853217
DOIs
StatePublished - 1 Jan 2021

Publication series

NameProgress in Molecular Biology and Translational Science
Volume179
ISSN (Print)1877-1173
ISSN (Electronic)1878-0814

Keywords

  • Bacterial removal
  • CRISPR/Cas9 system
  • Clinical settings
  • Infectious disease

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology

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