TY - CHAP
T1 - CRISPR based bacterial genome editing and removal of pathogens
AU - Jothi, Ravi
AU - Karthika, Chandrasekar
AU - Kamaladevi, Arumugam
AU - Satish, Lakkakula
AU - Pandian, Shunmugiah Karutha
AU - Gowrishankar, Shanmugaraj
N1 - Funding Information:
The authors sincerely acknowledge UGC-SAP [Grant No. F.5-1/2018/DRS-II (SAP-II)], DST-FIST [Grant No. SR/FST/LSI-639/2015 (C)] and DST PURSE [Grant No. SR/PURSE Phase 2/38 (G)] for rendering instrumentation & infrastructure facilities. SG gratefully acknowledges UGC for Start-Up Grant (Grant No. F.30-381/2017(BSR)/F·D Diary No. 2892), Alagappa University for AURF (Ref: ALU:AURF Start-up Grant: 2018)) and RUSA 2.0 [F.24-51/2014-U, Policy (TN Multi-Gen), Department of Education, Government of India].
Funding Information:
The authors sincerely acknowledge UGC-SAP [Grant No. F.5-1/2018/DRS-II (SAP-II)], DST-FIST [Grant No. SR/FST/LSI-639/2015 (C)] and DST PURSE [Grant No. SR/PURSE Phase 2/38 (G)] for rendering instrumentation & infrastructure facilities. SG gratefully acknowledges UGC for Start-Up Grant (Grant No. F.30-381/2017(BSR)/F?D Diary No. 2892), Alagappa University for AURF (Ref: ALU:AURF Start-up Grant: 2018)) and RUSA 2.0 [F.24-51/2014-U, Policy (TN Multi-Gen), Department of Education, Government of India].
Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - Engineering nucleases to achieve targeted genome editing has turned out to be a revolutionary means for manipulating the genetic content in diversified living organisms. For targeted genome editing, till to date, only three engineered nucleases exist viz. zinc finger nucleases, transcription activator–like effector nucleases and RNA-mediated nucleases (RGNs) (Cas nucleases) from the clustered regularly interspaced short palindromic repeat (CRISPR). Among, Cas9 nuclease has been considered as a simplest tool for efficient modification of endogenous genes in an extensive stretch of organisms, owing to its amenability to design guide RNA compatible to the sequence of new targets. Moreover, CRISPR/Cas system delivers a multipurpose RNA-guided DNA-targeting platform called as CRISPR interference (CRISPRi), as well as epigenetic modifications and high throughput screening in diverse organism including bacteria, all in a sequence explicit way. With these entire advancements, the present chapter illustrates the deployment of CRISPR/Cas9 in bacterial genome editing and removal of pathogens.
AB - Engineering nucleases to achieve targeted genome editing has turned out to be a revolutionary means for manipulating the genetic content in diversified living organisms. For targeted genome editing, till to date, only three engineered nucleases exist viz. zinc finger nucleases, transcription activator–like effector nucleases and RNA-mediated nucleases (RGNs) (Cas nucleases) from the clustered regularly interspaced short palindromic repeat (CRISPR). Among, Cas9 nuclease has been considered as a simplest tool for efficient modification of endogenous genes in an extensive stretch of organisms, owing to its amenability to design guide RNA compatible to the sequence of new targets. Moreover, CRISPR/Cas system delivers a multipurpose RNA-guided DNA-targeting platform called as CRISPR interference (CRISPRi), as well as epigenetic modifications and high throughput screening in diverse organism including bacteria, all in a sequence explicit way. With these entire advancements, the present chapter illustrates the deployment of CRISPR/Cas9 in bacterial genome editing and removal of pathogens.
KW - Bacterial removal
KW - Clinical settings
KW - CRISPR/Cas9 system
KW - Infectious disease
UR - http://www.scopus.com/inward/record.url?scp=85100124182&partnerID=8YFLogxK
U2 - 10.1016/bs.pmbts.2020.12.013
DO - 10.1016/bs.pmbts.2020.12.013
M3 - Chapter
C2 - 33785178
AN - SCOPUS:85100124182
SN - 9780323853217
T3 - Progress in Molecular Biology and Translational Science
SP - 77
EP - 92
BT - Reprogramming the Genome
A2 - Singh, Vijai
PB - Elsevier B.V.
ER -
