CRISPR based development of RNA editing and the diagnostic platform

Lakkakula Satish, Gunamalai Lavanya, Thirupathi Kasthuri, Aruchamy Kalaivaani, Sasanala Shamili, Pandiyan Muthuramalingam, Shanmugaraj Gowrishankar, Shunmugiah Karutha Pandian, Vijai Singh, Yaron Sitrit, Ariel Kushmaro

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Clustered Regularly Interspersed Short Palindromic Repeat-CRISPR-Associated (CRISPR-Cas) system has improved the ability to edit and control gene expression as desired. Genome editing approaches are currently leading the biomedical research with improved focus on direct nuclease dependent editing. So far, the research was predominantly intended on genome editing over the DNA level, recent adapted techniques are initiating to secure momentum through their proficiency to provoke modifications in RNA sequence. Integration of this system besides to lateral flow method allows reliable, quick, sensitive, precise and inexpensive diagnostic. These interesting methods illustrate only a small proportion of what is technically possible for this novel technology, but several technological obstacles need to be overcome prior to the CRISPR-Cas genome editing system can meet its full ability. This chapter covers the particulars on recent advances in CRISPR-Cas9 genome editing technology including diagnosis and technical advancements, followed by molecular mechanism of CRISPR-based RNA editing and diagnostic tools and types, and CRISPR-Cas-based biosensors.

Original languageEnglish
Title of host publicationReprogramming the Genome
Subtitle of host publicationApplications of CRISPR-Cas in Non-mammalian Systems Part A
EditorsVijai Singh
PublisherElsevier B.V.
Number of pages43
ISBN (Print)9780323853217
StatePublished - 1 Jan 2021

Publication series

NameProgress in Molecular Biology and Translational Science
ISSN (Print)1877-1173
ISSN (Electronic)1878-0814


  • Biosensors
  • CRISPR-Cas system
  • Genome editing
  • Non-coding RNA
  • Targeted gene repair

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology


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