CRISPR-Cas9 system for fungi genome engineering toward industrial applications

Lakkakula Satish; Sasanala Shamili; Balasubramanian C. Muthubharathi; Stanislaus Antony Ceasar; Ariel Kushmaro; Vijai Singh; Yaron Sitrit

doi:10.1016/B978-0-12-818140-9.00006-4

CRISPR-Cas9 system for fungi genome engineering toward industrial applications

Lakkakula Satish, Sasanala Shamili, Balasubramanian C. Muthubharathi, Stanislaus Antony Ceasar, Ariel Kushmaro, Vijai Singh, Yaron Sitrit

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

7 Scopus citations

Abstract

The discovery of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated protein 9 (Cas9) system has emerged as a prominent system for genome engineering in diverse organisms. Biotechnological and industrial production of foods, beverages, chemicals, proteins and fuels is dependent on effective production systems, commonly utilizing the genetically modified organisms. CRISPR-Cas9 approach has been used for genome editing of wide range of fungi and yeast for industrial applications. It has enabled significant advances in mycology research by hastening the growth of study models for those aiming to discover and validate novel compounds. This chapter aims to bring a comprehensive detail on application of CRISPR-Cas9 mediated genome editing in different fungi toward industrial applications.

Original language	English
Title of host publication	Genome Engineering via CRISPR-Cas9 System
Publisher	Elsevier
Pages	69-81
Number of pages	13
ISBN (Electronic)	9780128181409
ISBN (Print)	9780128181416
DOIs	https://doi.org/10.1016/B978-0-12-818140-9.00006-4
State	Published - 1 Jan 2020

Keywords

CRISPR-Cas9
Fungi
Genetic innovations
Genome editing
Industrial applications
sgRNA

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/B978-0-12-818140-9.00006-4

Cite this

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abstract = "The discovery of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated protein 9 (Cas9) system has emerged as a prominent system for genome engineering in diverse organisms. Biotechnological and industrial production of foods, beverages, chemicals, proteins and fuels is dependent on effective production systems, commonly utilizing the genetically modified organisms. CRISPR-Cas9 approach has been used for genome editing of wide range of fungi and yeast for industrial applications. It has enabled significant advances in mycology research by hastening the growth of study models for those aiming to discover and validate novel compounds. This chapter aims to bring a comprehensive detail on application of CRISPR-Cas9 mediated genome editing in different fungi toward industrial applications.",

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AU - Satish, Lakkakula

AU - Shamili, Sasanala

AU - Muthubharathi, Balasubramanian C.

AU - Ceasar, Stanislaus Antony

AU - Kushmaro, Ariel

AU - Singh, Vijai

AU - Sitrit, Yaron

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AB - The discovery of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated protein 9 (Cas9) system has emerged as a prominent system for genome engineering in diverse organisms. Biotechnological and industrial production of foods, beverages, chemicals, proteins and fuels is dependent on effective production systems, commonly utilizing the genetically modified organisms. CRISPR-Cas9 approach has been used for genome editing of wide range of fungi and yeast for industrial applications. It has enabled significant advances in mycology research by hastening the growth of study models for those aiming to discover and validate novel compounds. This chapter aims to bring a comprehensive detail on application of CRISPR-Cas9 mediated genome editing in different fungi toward industrial applications.

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KW - Fungi

KW - Genetic innovations

KW - Genome editing

KW - Industrial applications

KW - sgRNA

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BT - Genome Engineering via CRISPR-Cas9 System

PB - Elsevier

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CRISPR-Cas9 system for fungi genome engineering toward industrial applications

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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