Crystallization and preliminary crystallographic analysis of the Magnetospirillum magneticum AMB - 1 and M. gryphiswaldense MSR-1 magnetosome-associated proteins MamA

Natalie Zeytuni, Raz Zarivach

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

MamA is a unique magnetosome-associated protein that is predicted to contain six sequential tetratricopeptide-repeat (TPR) motifs. The TPR structural motif serves as a template for protein-protein interactions and mediates the assembly of multi-protein complexes. Here, the crystallization and preliminary X-ray analysis of recombinant and purified Magnetospirillum magneticum and M. gryphiswaldense MamA are reported for the first time. M. gryphiswaldense MamAΔ41 crystallized in the tetragonal space group P412 12 or P43212, with unit-cell parameters a = b = 58.88, c = 144.09 Å. M. magneticum MamAΔ41 crystallized in the orthorhombic space group P212121, with unit-cell parameters a = 44.75, b = 76.19, c = 105.05 Å. X-ray diffraction data were collected to resolutions of 2.0 and 1.95 Å, respectively.

Original languageEnglish
Pages (from-to)824-827
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number7
DOIs
StatePublished - 15 Jul 2010

Keywords

  • Magnetospirillum gryphiswaldense MSR-1
  • Magnetospirillum magneticum AMB-1
  • MamA
  • magnetosome-associated proteins

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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