Crystallization and preliminary crystallographic analysis of the Magnetospirillum magneticum AMB - 1 and M. gryphiswaldense MSR-1 magnetosome-associated proteins MamA

MamA is a unique magnetosome-associated protein that is predicted to contain six sequential tetratricopeptide-repeat (TPR) motifs. The TPR structural motif serves as a template for protein-protein interactions and mediates the assembly of multi-protein complexes. Here, the crystallization and preliminary X-ray analysis of recombinant and purified Magnetospirillum magneticum and M. gryphiswaldense MamA are reported for the first time. M. gryphiswaldense MamAΔ41 crystallized in the tetragonal space group P4₁2 ₁2 or P4₃2₁2, with unit-cell parameters a = b = 58.88, c = 144.09 Å. M. magneticum MamAΔ41 crystallized in the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 44.75, b = 76.19, c = 105.05 Å. X-ray diffraction data were collected to resolutions of 2.0 and 1.95 Å, respectively.