@inbook{52ed3200de8d4c87bc1ca49c364f2ad6,
title = "Culturing Three Dimensional MDCK cells for Analyzing Intracellular Dynamics",
abstract = "Epithelial cells grown in three dimensional (3D) cultures of extracellular matrix differentiate into a multicellular structure of polarized cells. This process shares many characteristics with the physiological development of an epithelial tissue and the formation of polarity in epithelial cells. Imaging 3D cultures of polarized epithelial cells is therefore a powerful tool to study epithelial architecture and morphogenesis under close to physiological conditions. The new generation of confocal microscopes allows live cell imaging of fluorescently tagged molecules in these cultures. This opens up new opportunities for studying how molecules behave and are distinguished asymmetrically within a 3D setting. This unit discusses technical aspects for culturing and imaging MDCK 3D culture for both fixed 3D cultures and live cell imaging",
keywords = "3d culture imaging, 3d cultures, epithelial cyst, live cell imaging, mdck cells, mdck cyst",
author = "Natalie Elia and Jennifer Lippincott-schwartz",
year = "2009",
language = "English",
isbn = "0471143030",
series = "Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]",
booktitle = "Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]",
}