Cutaneous leishmaniasis serodiagnosis by immunoperoxidase assay

D. Halevy, B. Sarov, I. Sarov, J. El-On

Research output: Contribution to journalArticlepeer-review

Abstract

Immunoperoxidase assay was used for the determination of serum-specific anti-leishmanial IgG antibodies in 65 patients with cutaneous leishmaniasis (CL), in 5 with visceral leishmaniasis (VL) and in 84 controls. A significant difference was observed between CL and VL sera and the control sera when either Leishmania major, L. donovani or L. aethiopica intact promastigotes were used as antigens. CL patients showed similar activity against L. major and L. donovani antigen (titers 4-64 and <2-64 respectively), whereas VL patients showed a higher activity with the homologous antigen L. donovani (titers 128-2,048). A high and significant correlation was demonstrated between immunoperoxidase assay, immunofluorescent assay and radioimmunoassay. No cross-reactivity was found with sera from malaria patients.

Original languageEnglish
Pages (from-to)485-490
Number of pages6
JournalIsrael Journal of Medical Sciences
Volume26
Issue number9
StatePublished - 1 Jan 1990

Keywords

  • Cutaneous leishmaniasis
  • Immunofluorescent assay
  • Immunoperoxidase assay
  • Serodiagnosis

Fingerprint

Dive into the research topics of 'Cutaneous leishmaniasis serodiagnosis by immunoperoxidase assay'. Together they form a unique fingerprint.

Cite this