Cystine accumulation impairs glucose transport in LLC-PK1 cells

A. Ben-Nun, N. Bashan, R. Potashnik, A. Moran, Arie Moran

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4 Scopus citations

Abstract

We studied the effect of lysosomal loading with cystine (by incubation with cystine dimethyl ester, CDME) on the functions of renal epithelial cells in culture (LLC- PKi). The concentrating capacity (the ratio of the intra- and extracellular concentrations) of the nonmetabolized glucose analog, a-methylglucoside (AMG), was reduced after incubation with CDME in a dose- and time-dependent manner. Consistent with these findings was a 30% reduction in the number of the sodium-coupled D-glucose transporters assessed by phlorizin binding. Since the impairment in glucose transport was less effective with other amino acid methyl ester analogues, LLC-PK cells treated with CDME can be used to study the cellular mechanisms responsible for the impairment of kidney function in cystine accumulation diseases, e.g., cystinosis.

Original languageEnglish
Pages (from-to)294-304
Number of pages11
JournalCellular Physiology and Biochemistry
Volume1
Issue number5-6
DOIs
StatePublished - 1 Jan 1991

Keywords

  • Cystinosis
  • LLC-PK
  • Lysosomal cystine accumulation
  • Phlorizin binding
  • Sodium-coupled glucose transport

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