TY - JOUR
T1 - Cystine dimethyl ester reduces the forces driving sodium-dependent transport in LLC-PK1 cells
AU - Ben-Nun, A.
AU - Bashan, N.
AU - Potashnik, R.
AU - Cohen-Luria, R.
AU - Moran, A.
PY - 1992/1/1
Y1 - 1992/1/1
N2 - Cystinosis is an inherited metabolic disease characterized by accumulation of lysosomal cystine and renal impairment. In an attempt to better understand the link between cystine accumulation and renal functions, we studied the effects of cystine loading on the Na+-H+ antiporter and the sodium pump in renal epithelial cells (LLC-PK1) in culture. Incubation of LLC-PK1 with 1 mM cystine dimethyl ester (CDME) for 48 h caused lysosomal cystine loading and reduced by 22 ± 2% the maximal velocity of sodium-hydrogen antiport with no significant change in the affinity of sodium for the transporter. Rubidium influx decreased to 46 ± 5% of control. Ouabain binding experiments revealed a 10% reduction in the number of Na+-K+-ATPase units in the intact cells. Na+-K+-ATPase activity in the particulate fraction of the cells homogenate declined to 50 ± 7.5% of controls. No significant change was observed in the activity of ouabain-insensitive phosphatases. The intracellular concentration of sodium increased from 20.6 ± 3.7 to 64.8 ± 10 mM, and potassium concentration decreased from 103 ± 6 to 80 ± 13 mM. In addition to the observed reduction in the sodium gradient and in agreement with the reduction in the intracellular potassium concentration, the membrane potential changed from -80.8 ± 7.5 to -69.9 ± 7.0 mV. The results suggest that intracellular accumulation of cystine is associated with reduction in the number and the activity of membrane transporters. The consequence of the changes in the activity of Na+-K+-ATPase is a reduction in the electrochemical forces that drive transport in the renal cells tested. This mechanism may be the underlying reason for the development of Fanconi's syndrome in cystinotic patients.
AB - Cystinosis is an inherited metabolic disease characterized by accumulation of lysosomal cystine and renal impairment. In an attempt to better understand the link between cystine accumulation and renal functions, we studied the effects of cystine loading on the Na+-H+ antiporter and the sodium pump in renal epithelial cells (LLC-PK1) in culture. Incubation of LLC-PK1 with 1 mM cystine dimethyl ester (CDME) for 48 h caused lysosomal cystine loading and reduced by 22 ± 2% the maximal velocity of sodium-hydrogen antiport with no significant change in the affinity of sodium for the transporter. Rubidium influx decreased to 46 ± 5% of control. Ouabain binding experiments revealed a 10% reduction in the number of Na+-K+-ATPase units in the intact cells. Na+-K+-ATPase activity in the particulate fraction of the cells homogenate declined to 50 ± 7.5% of controls. No significant change was observed in the activity of ouabain-insensitive phosphatases. The intracellular concentration of sodium increased from 20.6 ± 3.7 to 64.8 ± 10 mM, and potassium concentration decreased from 103 ± 6 to 80 ± 13 mM. In addition to the observed reduction in the sodium gradient and in agreement with the reduction in the intracellular potassium concentration, the membrane potential changed from -80.8 ± 7.5 to -69.9 ± 7.0 mV. The results suggest that intracellular accumulation of cystine is associated with reduction in the number and the activity of membrane transporters. The consequence of the changes in the activity of Na+-K+-ATPase is a reduction in the electrochemical forces that drive transport in the renal cells tested. This mechanism may be the underlying reason for the development of Fanconi's syndrome in cystinotic patients.
KW - lysosomal cystine accumulation
KW - sodium- potassium-adenosinetriphosphatase
KW - sodium-hydrogen exchanger
UR - http://www.scopus.com/inward/record.url?scp=0026782704&partnerID=8YFLogxK
U2 - 10.1152/ajpcell.1992.263.2.c516
DO - 10.1152/ajpcell.1992.263.2.c516
M3 - Article
C2 - 1325121
AN - SCOPUS:0026782704
SN - 0363-6143
VL - 263
SP - C516-C520
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
IS - 2 32-2
ER -