Cytokine mRNA Expression in Pneumococcal Carriage, Pneumonia, and Sepsis in Young Mice

Eduard Ling, Galia Feldman, Ron Dagan, Yaffa Mizrachi-Nebenzahl

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


We studied cytokine mRNA expression in Streptococcus pneumoniae carriage, pneumonia, and sepsis in 3-week-old, inbred C57BL/6 and BALB/c mice. Mice were inoculated intranasally with S. pneumoniae serotype 6B, 14, or 3. Survival, bacterial load in the nasopharynx (NP) and lungs, and mRNA levels of tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, interleukin (IL)-10, and IL-12 in the spleen were analyzed. No baseline mRNA expression levels were found, except for TNF-α in C57BL/6 mice. Serotype 6B caused NP colonization only, a significant (P < .05) increase in the levels of TNF-α, and induction of TGF-β and IL-12 mRNA. Serotype 14 caused NP and nonlethal lung colonization and induction of TGF-β, IL-10, and IL-12. Serotype 3 caused NP colonization, pneumonia, 35% mortality, and no alteration in the mRNA expression of tested cytokines. Although activation of the immune system culminated in nonlethal disease, evasion of the immune system was associated with detrimental disease.

Original languageEnglish
Pages (from-to)1752-1756
Number of pages5
JournalJournal of Infectious Diseases
Issue number11
StatePublished - 1 Dec 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases


Dive into the research topics of 'Cytokine mRNA Expression in Pneumococcal Carriage, Pneumonia, and Sepsis in Young Mice'. Together they form a unique fingerprint.

Cite this