TY - JOUR
T1 - Cytokine mRNA Expression in Pneumococcal Carriage, Pneumonia, and Sepsis in Young Mice
AU - Ling, Eduard
AU - Feldman, Galia
AU - Dagan, Ron
AU - Mizrachi-Nebenzahl, Yaffa
PY - 2003/12/1
Y1 - 2003/12/1
N2 - We studied cytokine mRNA expression in Streptococcus pneumoniae carriage, pneumonia, and sepsis in 3-week-old, inbred C57BL/6 and BALB/c mice. Mice were inoculated intranasally with S. pneumoniae serotype 6B, 14, or 3. Survival, bacterial load in the nasopharynx (NP) and lungs, and mRNA levels of tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, interleukin (IL)-10, and IL-12 in the spleen were analyzed. No baseline mRNA expression levels were found, except for TNF-α in C57BL/6 mice. Serotype 6B caused NP colonization only, a significant (P < .05) increase in the levels of TNF-α, and induction of TGF-β and IL-12 mRNA. Serotype 14 caused NP and nonlethal lung colonization and induction of TGF-β, IL-10, and IL-12. Serotype 3 caused NP colonization, pneumonia, 35% mortality, and no alteration in the mRNA expression of tested cytokines. Although activation of the immune system culminated in nonlethal disease, evasion of the immune system was associated with detrimental disease.
AB - We studied cytokine mRNA expression in Streptococcus pneumoniae carriage, pneumonia, and sepsis in 3-week-old, inbred C57BL/6 and BALB/c mice. Mice were inoculated intranasally with S. pneumoniae serotype 6B, 14, or 3. Survival, bacterial load in the nasopharynx (NP) and lungs, and mRNA levels of tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, interleukin (IL)-10, and IL-12 in the spleen were analyzed. No baseline mRNA expression levels were found, except for TNF-α in C57BL/6 mice. Serotype 6B caused NP colonization only, a significant (P < .05) increase in the levels of TNF-α, and induction of TGF-β and IL-12 mRNA. Serotype 14 caused NP and nonlethal lung colonization and induction of TGF-β, IL-10, and IL-12. Serotype 3 caused NP colonization, pneumonia, 35% mortality, and no alteration in the mRNA expression of tested cytokines. Although activation of the immune system culminated in nonlethal disease, evasion of the immune system was associated with detrimental disease.
UR - http://www.scopus.com/inward/record.url?scp=0347517723&partnerID=8YFLogxK
U2 - 10.1086/379371
DO - 10.1086/379371
M3 - Article
C2 - 14639547
AN - SCOPUS:0347517723
SN - 0022-1899
VL - 188
SP - 1752
EP - 1756
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 11
ER -
