Cytotoxic and apoptotic inducing activity of Amoora rohituka leaf extracts in human breast cancer cells

Rajesh Kumar Singh, Amit Ranjan, Akhileshwar Kumar Srivastava, Monika Singh, Anil Kumar Shukla, Neelam Atri, Anurag Mishra, Anil Kumar Singh, Santosh Kumar Singh

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Background: Amoora rohituka is described in Ayurveda, an Indian traditional system of medicine for management of disorders of blood, diseases of eye, helminthiasis disease, ulcer, liver disorders and splenomegaly. However, the leaves were not reported to have anticancer properties till date. Objective: This study was carried out to evaluate the cytotoxic potential of leaf extracts of Amoora rohituka. Materials and methods: The leaves powder was macerated in petroleum ether, ethyl acetate and methanol and evaluated their anticancer activities in vitro. The phytochemical constituents of the active (ethyl acetate) extract were screened by FTIR analysis and phytochemical screening methods. Results: The ethyl acetate extract (RLEA) showed the presence of alkaloids, flavonoids, steroids, tannins, saponins and terpenoids. The RLEA exhibited high cytotoxic effect against human breast cancer cells, MCF-7 (IC50 = 9.81 μg/mL) and induced apoptosis by altering nuclear morphology and DNA laddering. Wound healing assays explained the potency of extract to decrease the cell migration. Conclusion: The extract of Amoora rohituka leaves exhibited anticancer activity with less toxicity and it could be used for development of alternative drugs in the treatment of human breast cancer.

Original languageEnglish
Pages (from-to)383-390
Number of pages8
JournalJournal of Ayurveda and Integrative Medicine
Volume11
Issue number4
DOIs
StatePublished - 1 Oct 2020

Keywords

  • Apoptosis
  • Breast cancer
  • Cell migration
  • FTIR
  • HPTLC
  • Traditional medicine

ASJC Scopus subject areas

  • Drug Discovery
  • Complementary and alternative medicine

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