TY - JOUR
T1 - d-Glutamate production by stressed Escherichia coli gives a clue for the hypothetical induction mechanism of the ALS disease
AU - Monselise, Edna Ben Izhak
AU - Vyazmensky, Maria
AU - Scherf, Tali
AU - Batushansky, Albert
AU - Fishov, Itzhak
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/12/1
Y1 - 2024/12/1
N2 - In the search for the origin of Amyotrophic Lateral Sclerosis disease (ALS), we hypothesized earlier (Monselise, 2019) that d-amino acids produced by stressed microbiome may serve as inducers of the disease development. Many examples of d-amino acid accumulation under various stress conditions were demonstrated in prokaryotic and eukaryotic cells. In this work, wild-type Escherichia coli, members of the digestive system, were subjected to carbon and nitrogen starvation stress. Using NMR and LC–MS techniques, we found for the first time that d-glutamate accumulated in the stressed bacteria but not in control cells. These results together with the existing knowledge, allow us to suggest a new insight into the pathway of ALS development: d-glutamate, produced by the stressed microbiome, induces neurobiochemical miscommunication setting on C1q of the complement system. Proving this insight may have great importance in preventive medicine of such MND modern-age diseases as ALS, Alzheimer, and Parkinson.
AB - In the search for the origin of Amyotrophic Lateral Sclerosis disease (ALS), we hypothesized earlier (Monselise, 2019) that d-amino acids produced by stressed microbiome may serve as inducers of the disease development. Many examples of d-amino acid accumulation under various stress conditions were demonstrated in prokaryotic and eukaryotic cells. In this work, wild-type Escherichia coli, members of the digestive system, were subjected to carbon and nitrogen starvation stress. Using NMR and LC–MS techniques, we found for the first time that d-glutamate accumulated in the stressed bacteria but not in control cells. These results together with the existing knowledge, allow us to suggest a new insight into the pathway of ALS development: d-glutamate, produced by the stressed microbiome, induces neurobiochemical miscommunication setting on C1q of the complement system. Proving this insight may have great importance in preventive medicine of such MND modern-age diseases as ALS, Alzheimer, and Parkinson.
KW - Eukaryotic and prokaryotic communication
KW - Evolutionary approach
KW - Mitochondria
KW - d-glutamate and d-glutamate racemase
UR - http://www.scopus.com/inward/record.url?scp=85200540969&partnerID=8YFLogxK
U2 - 10.1038/s41598-024-68645-8
DO - 10.1038/s41598-024-68645-8
M3 - Article
AN - SCOPUS:85200540969
SN - 2045-2322
VL - 14
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 18247
ER -