De novo Semi-Synthetic Platform for Monitoring Protein degradation in Live Cells

Mahdi Hasan; Deepanjan Panda; Guy Mann; Ashraf Brik

doi:10.1002/cbic.202300731

De novo Semi-Synthetic Platform for Monitoring Protein degradation in Live Cells

Mahdi Hasan, Deepanjan Panda, Guy Mann, Ashraf Brik

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We designed a platform for monitoring the degradation of exogenous proteins in live cells. We engineered a semi-synthetic platform, which consists of Enhanced Green Fluorescent Protein tagged with SpyCatcher to enable its conjugation to a SpyTag peptide bearing a Von Hippel–Lindau E3 ligand, which was delivered to live cells to promote its degradation. This platform lays the ground for studying the degradation of endogenous proteins equipped with SpyTag and for tracking the degradation of post-translationally modified proteins in live cells.

Original language	English
Article number	e202300731
Journal	ChemBioChem
Volume	25
Issue number	3
DOIs	https://doi.org/10.1002/cbic.202300731
State	Published - 1 Feb 2024
Externally published	Yes

Keywords

Bead loading delivery
Peptide synthesis
Proteasomal degradation
SpyTag/SpyCatcher

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.202300731

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abstract = "We designed a platform for monitoring the degradation of exogenous proteins in live cells. We engineered a semi-synthetic platform, which consists of Enhanced Green Fluorescent Protein tagged with SpyCatcher to enable its conjugation to a SpyTag peptide bearing a Von Hippel–Lindau E3 ligand, which was delivered to live cells to promote its degradation. This platform lays the ground for studying the degradation of endogenous proteins equipped with SpyTag and for tracking the degradation of post-translationally modified proteins in live cells.",

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author = "Mahdi Hasan and Deepanjan Panda and Guy Mann and Ashraf Brik",

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AU - Mann, Guy

AU - Brik, Ashraf

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AB - We designed a platform for monitoring the degradation of exogenous proteins in live cells. We engineered a semi-synthetic platform, which consists of Enhanced Green Fluorescent Protein tagged with SpyCatcher to enable its conjugation to a SpyTag peptide bearing a Von Hippel–Lindau E3 ligand, which was delivered to live cells to promote its degradation. This platform lays the ground for studying the degradation of endogenous proteins equipped with SpyTag and for tracking the degradation of post-translationally modified proteins in live cells.

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KW - Peptide synthesis

KW - Proteasomal degradation

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De novo Semi-Synthetic Platform for Monitoring Protein degradation in Live Cells

Abstract

Keywords

ASJC Scopus subject areas

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