Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex

Erik W. Debler, Gunnar F. Kaufmann, Michael M. Meijler, Andreas Heine, Jenny M. Mee, Goran Pljevaljčić, Angel J. Di Bilio, Peter G. Schultz, David P. Millar, Kim D. Janda, Ian A. Wilson, Harry B. Gray, Richard A. Lerner

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel π-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.

Original languageEnglish
Pages (from-to)1232-1235
Number of pages4
Issue number5867
StatePublished - 29 Feb 2008

ASJC Scopus subject areas

  • General


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