Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex

Erik W. Debler; Gunnar F. Kaufmann; Michael M. Meijler; Andreas Heine; Jenny M. Mee; Goran Pljevaljčić; Angel J. Di Bilio; Peter G. Schultz; David P. Millar; Kim D. Janda; Ian A. Wilson; Harry B. Gray; Richard A. Lerner

doi:10.1126/science.1153445

Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex

Erik W. Debler, Gunnar F. Kaufmann, Michael M. Meijler, Andreas Heine, Jenny M. Mee, Goran Pljevaljčić, Angel J. Di Bilio, Peter G. Schultz, David P. Millar, Kim D. Janda, Ian A. Wilson, Harry B. Gray, Richard A. Lerner

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel π-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.

Original language	English
Pages (from-to)	1232-1235
Number of pages	4
Journal	Science
Volume	319
Issue number	5867
DOIs	https://doi.org/10.1126/science.1153445
State	Published - 29 Feb 2008

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title = "Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex",

abstract = "The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel π-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.",

author = "Debler, {Erik W.} and Kaufmann, {Gunnar F.} and Meijler, {Michael M.} and Andreas Heine and Mee, {Jenny M.} and Goran Pljevalj{\v c}i{\'c} and {Di Bilio}, {Angel J.} and Schultz, {Peter G.} and Millar, {David P.} and Janda, {Kim D.} and Wilson, {Ian A.} and Gray, {Harry B.} and Lerner, {Richard A.}",

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volume = "319",

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T1 - Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex

AU - Debler, Erik W.

AU - Kaufmann, Gunnar F.

AU - Meijler, Michael M.

AU - Heine, Andreas

AU - Mee, Jenny M.

AU - Pljevaljčić, Goran

AU - Di Bilio, Angel J.

AU - Schultz, Peter G.

AU - Millar, David P.

AU - Janda, Kim D.

AU - Wilson, Ian A.

AU - Gray, Harry B.

AU - Lerner, Richard A.

PY - 2008/2/29

Y1 - 2008/2/29

N2 - The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel π-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.

AB - The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel π-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.

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DO - 10.1126/science.1153445

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JO - Science

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Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex

Abstract

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