Degradation of brominated organic compounds (Flame retardants) by a four-strain consortium isolated from contaminated groundwater

Noa Balaban, Faina Gelman, Alicia A. Taylor, Sharon L. Walker, Anat Bernstein, Zeev Ronen

Research output: Contribution to journalArticlepeer-review

Abstract

Biodegradation of pollutants in the environment is directly affected by microbial communities and pollutant mixture at the site. Lab experiments using bacterial consortia and substrate mixtures are required to increase our understanding of these processes in the environment. One of the deficiencies of working with environmental cultures is the inability to culture and identify the active strains while knowing they are representative of the original environment. In the present study, we tested the aerobic microbial degradation of two brominated flame retardants, tribromo-neopentyl alcohol (TBNPA) and dibromo neopentyl glycol (DBNPG), by an assembled bacterial consortium of four strains. The four strains were isolated and plate-cultured from a consortium enriched from the impacted groundwater underlying the Neot Hovav industrial area (Negev, Israel), in which TBNPA and DBNPG are abundant pollutants. Total degradation (3-7 days) occurred only when the four-strain consortium was incubated together (25 °C; pH −7.2) with an additional carbon source, as both compounds were not utilized as such. Bacterial growth was found to be the limiting factor. A dual carbon-bromine isotope analysis was used to corroborate the claim that the isolated strains were responsible for the degradation in the original enriched consortium, thus ensuring that the isolated four-strain microbial consortium is representative of the actual environmental enrichment.

Original languageEnglish
Article number6263
JournalApplied Sciences (Switzerland)
Volume11
Issue number14
DOIs
StatePublished - 2 Jul 2021

Keywords

  • Assembled microbial consortium
  • Brominated flame retardants
  • Isotopic fractionation
  • Simultaneous utilization
  • Substrate mixtures
  • Syntrophy

ASJC Scopus subject areas

  • Materials Science (all)
  • Instrumentation
  • Engineering (all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes

Fingerprint

Dive into the research topics of 'Degradation of brominated organic compounds (Flame retardants) by a four-strain consortium isolated from contaminated groundwater'. Together they form a unique fingerprint.

Cite this