DEGRADATION OF THE CELL‐WALL POLYSACCHARIDE OF PORPHYRIDIUM SP. (RHODOPHYTA) BY MEANS OF ENZYMATIC ACTIVITY OF ITS PREDATOR, GYMNODINIUM SP. (PYRROPHYTA)

Bracha Simon, Shimona Geresh, Shoshana (Malis) Arad

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The dinoflagellate Gymnodinium sp., which preys specifically on cells of the red microalga Porphyridium sp., possesses enzymes that degrade exocellular polysaccharides of the Porphyridium sp. A crude extract of Gymnodinium sp. was applied to this polysaccharide, and the degradation products were characterized by charge and size separations. Charge separation revealed the presence of a fraction that was not found in the native polysaccharide. This fraction, which was eluted from an anion‐exchange resin with water alone, was composed mostly of glucose and xylose (in a 1:1 weight ratio). Size separation of the degradation products revealed three fractions; the molecular weight of the main one was 5 × 106 daltons, whereas that of the native polysaccharide was 7 × 106 daltons. The carbohydrate composition of these fractions was determined. Although the main product of degradation had a relatively high molecular weight, its viscosity was significantly reduced relative to the native polysaccharide. Additional enzymatic degradation is required for further exploration of the structure of the exocellular polymer of Porphyridium sp.

Original languageEnglish
Pages (from-to)460-465
Number of pages6
JournalJournal of Phycology
Volume28
Issue number4
DOIs
StatePublished - 1 Jan 1992

Keywords

  • Gymnodinium
  • Porphyridium sp.
  • Pyrrophyta
  • Rhodophyta
  • extracellular mucilage
  • microalgae
  • sulfated polysaccharides

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

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