Depth of immersion of fluorescent chromophores in biomembranes studied by quenching with nitroxide radical

N. V. Strashnikova; N. Medvedeva; G. I. Likhtenshtein

doi:10.1016/S0165-022X(00)00144-5

Depth of immersion of fluorescent chromophores in biomembranes studied by quenching with nitroxide radical

N. V. Strashnikova, N. Medvedeva, G. I. Likhtenshtein

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A novel method has been developed for measuring depth of immersion of a fluorescent chromophore in biological matrices, such as biomembranes. The method is based on dynamic quenching of chromophore fluorescence by a nitroxide probe freely diffused in solution. Theoretical considerations and experimental evidences relating to the method are discussed. The proposed method was applied to investigation of lecithin liposomes and membranes from Bacillus subtilis modified by the photochrome-fluorescent probe 4,4′-dimethylaminocyanostilbene.

Original language	English
Pages (from-to)	43-60
Number of pages	18
Journal	Journal of Biochemical and Biophysical Methods
Volume	48
Issue number	1
DOIs	https://doi.org/10.1016/S0165-022X(00)00144-5
State	Published - 28 Mar 2001

Keywords

Biomembrane
Depth of immersion
Fluorophores
Nitroxides

ASJC Scopus subject areas

Biophysics
Biochemistry

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10.1016/S0165-022X(00)00144-5

Cite this

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title = "Depth of immersion of fluorescent chromophores in biomembranes studied by quenching with nitroxide radical",

abstract = "A novel method has been developed for measuring depth of immersion of a fluorescent chromophore in biological matrices, such as biomembranes. The method is based on dynamic quenching of chromophore fluorescence by a nitroxide probe freely diffused in solution. Theoretical considerations and experimental evidences relating to the method are discussed. The proposed method was applied to investigation of lecithin liposomes and membranes from Bacillus subtilis modified by the photochrome-fluorescent probe 4,4′-dimethylaminocyanostilbene.",

keywords = "Biomembrane, Depth of immersion, Fluorophores, Nitroxides",

author = "Strashnikova, {N. V.} and N. Medvedeva and Likhtenshtein, {G. I.}",

note = "Funding Information: The authors thank Dr. I. Fishov and E. Klyman for generous help in growing microorganisms, PhD student V. Papper for the synthesis of stilbene probe and Dr. A. Shames for taking ESR spectra. Financial support from the James Frank Binational German-Israel Program in Laser-Matter Interaction is gratefully acknowledged. ",

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doi = "10.1016/S0165-022X(00)00144-5",

language = "English",

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T1 - Depth of immersion of fluorescent chromophores in biomembranes studied by quenching with nitroxide radical

AU - Strashnikova, N. V.

AU - Medvedeva, N.

AU - Likhtenshtein, G. I.

N1 - Funding Information: The authors thank Dr. I. Fishov and E. Klyman for generous help in growing microorganisms, PhD student V. Papper for the synthesis of stilbene probe and Dr. A. Shames for taking ESR spectra. Financial support from the James Frank Binational German-Israel Program in Laser-Matter Interaction is gratefully acknowledged.

PY - 2001/3/28

Y1 - 2001/3/28

N2 - A novel method has been developed for measuring depth of immersion of a fluorescent chromophore in biological matrices, such as biomembranes. The method is based on dynamic quenching of chromophore fluorescence by a nitroxide probe freely diffused in solution. Theoretical considerations and experimental evidences relating to the method are discussed. The proposed method was applied to investigation of lecithin liposomes and membranes from Bacillus subtilis modified by the photochrome-fluorescent probe 4,4′-dimethylaminocyanostilbene.

AB - A novel method has been developed for measuring depth of immersion of a fluorescent chromophore in biological matrices, such as biomembranes. The method is based on dynamic quenching of chromophore fluorescence by a nitroxide probe freely diffused in solution. Theoretical considerations and experimental evidences relating to the method are discussed. The proposed method was applied to investigation of lecithin liposomes and membranes from Bacillus subtilis modified by the photochrome-fluorescent probe 4,4′-dimethylaminocyanostilbene.

KW - Biomembrane

KW - Depth of immersion

KW - Fluorophores

KW - Nitroxides

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U2 - 10.1016/S0165-022X(00)00144-5

DO - 10.1016/S0165-022X(00)00144-5

M3 - Article

AN - SCOPUS:0035962067

SN - 0165-022X

VL - 48

SP - 43

EP - 60

JO - Journal of Biochemical and Biophysical Methods

JF - Journal of Biochemical and Biophysical Methods

IS - 1

ER -

Depth of immersion of fluorescent chromophores in biomembranes studied by quenching with nitroxide radical

Abstract

Keywords

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