Design and optimization of a near-field scanning optical microscope for imaging biological samples in liquid

Levi A. Gheber, Jeeseong Hwang, Michael Edidin

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

We describe a near-field scanning optical microscope capable of imaging biological samples in liquid. The microscope uses a straight optical fiber near-field probe and optical shear-force feedback for tip-sample distance regulation. Physical aspects of the design are discussed, and phenomena related to operation in liquid are revealed. Careful calibration of the instrument in air and in liquid is shown, and for the first time to our knowledge, near-field fluorescence images of a biological cell in liquid are presented.

Original languageEnglish
Pages (from-to)3574-3581
Number of pages8
JournalApplied Optics
Volume37
Issue number16
DOIs
StatePublished - 1 Jun 1998
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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