Design considerations and limitations for miniature spr devices

Since the last three decades, surface plasmon resonance (SPR) has provided new horizons to the chemical/biomedical industry as an optical tool for the analysis of molecular interactions and sensing applications. This chapter covers the design considerations and instrumental optics of a typical SPR device along with the interrogation schemes, various analytical methods to find the suitable SPR dip position, several parameters affecting the signal noise, and other basic units of the SPR system covering the flow channels and their properties along with sensogram of a generic SPR sensor at different sensing stages. The Minimum Hunt Method is based on determining the minimum of the SPR curve in several ways, such as looking at the minimum point of the reflection curve or finding a location where the first derivative vanishes. However, the required minimum location can be found by fitting a parabola (or higher-order polynomial function) to the portion of the curve around the dip and finding the vertex.