Design of an optical switch for studying conformational dynamics in individual molecules of GroEL

Gabriel A. Frank, Yakov Kipnis, Elena Smolensky, Shirley S. Daube, Amnon Horovitz, Gilad Haran

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We describe the design of an optical switch in the chaperonin GroEL that is opened and closed by its ATP- and cochaperonin GroES-driven conformational changes. The switch, based on a fluorophore and a quencher, is engineered into the single-ring variant of the chaperone, and shows dramatic modulation of its fluorescent intensity in response to the transition of the protein between its allosteric states. It, therefore, forms a sensitive probe for the dynamics of the allosteric transitions of this machine, both in the bulk and in single molecules.

Original languageEnglish
Pages (from-to)1339-1341
Number of pages3
JournalBioconjugate Chemistry
Volume19
Issue number7
DOIs
StatePublished - 1 Jan 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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