TY - JOUR
T1 - Detection of anti-ganglioside antibodies in Guillain-Barré syndrome and its variants by the agglutination assay
AU - Alaedini, Armin
AU - Briani, Chiara
AU - Wirguin, Itzhak
AU - Siciliano, Gabriele
AU - D'Avino, Carla
AU - Latov, Norman
N1 - Funding Information:
This study was supported, in part, by a grant from the NIH (NINCDS NS11766), and by generous donations from James and Linda Gardner, George Fisher, and Peter Bing.
PY - 2002/4/15
Y1 - 2002/4/15
N2 - Sera from 40 patients with Guillain-Barré syndrome (GBS), including the subtypes acute inflammatory demyelinating polyneuropathy (AIDP), acute motor axonal neuropathy (AMAN), acute motor and sensory axonal neuropathy (AMSAN), and Miller Fisher syndrome (MFS) were examined for the presence of anti-ganglioside antibodies using the ganglioside agglutination assay, and the enzyme-linked immunosorbent assay (ELISA). In the ELISA system, sera were tested for IgM and IgG antibodies to GM1, GM2, GD1a, GD1b, GT1b, and GQ1b gangliosides. Antibodies to gangliosides were detected in 21 (53%) of the GBS patients by agglutination assay and in 17 (43%) of the patients by ELISA. Some of the sera reacted with more than one ganglioside. Antibodies were not found in the control sera that were studied. The agglutination assay may be useful for rapid screening of GBS sera for antibodies to multiple gangliosides.
AB - Sera from 40 patients with Guillain-Barré syndrome (GBS), including the subtypes acute inflammatory demyelinating polyneuropathy (AIDP), acute motor axonal neuropathy (AMAN), acute motor and sensory axonal neuropathy (AMSAN), and Miller Fisher syndrome (MFS) were examined for the presence of anti-ganglioside antibodies using the ganglioside agglutination assay, and the enzyme-linked immunosorbent assay (ELISA). In the ELISA system, sera were tested for IgM and IgG antibodies to GM1, GM2, GD1a, GD1b, GT1b, and GQ1b gangliosides. Antibodies to gangliosides were detected in 21 (53%) of the GBS patients by agglutination assay and in 17 (43%) of the patients by ELISA. Some of the sera reacted with more than one ganglioside. Antibodies were not found in the control sera that were studied. The agglutination assay may be useful for rapid screening of GBS sera for antibodies to multiple gangliosides.
KW - Acute inflammatory demyelinating polyneuropathy
KW - Acute motor and sensory axonal neuropathy
KW - Acute motor axonal neuropathy
KW - Ganglioside antibodies
KW - Guillain-Barré syndrome
KW - Miller Fisher syndrome
UR - http://www.scopus.com/inward/record.url?scp=0037090437&partnerID=8YFLogxK
U2 - 10.1016/S0022-510X(02)00022-9
DO - 10.1016/S0022-510X(02)00022-9
M3 - Article
C2 - 11959155
AN - SCOPUS:0037090437
SN - 0022-510X
VL - 196
SP - 41
EP - 44
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
IS - 1-2
ER -