Detection of antibodies to varicella zoster virus by radioimmunoassay and enzyme immunoassay techniques

Maureen G. Friedman; Hava Haikin; Sophia Leventon-Kriss; Raya Joffe; Varda Goldstein; Israel Sarov

doi:10.1007/BF02121148

Detection of antibodies to varicella zoster virus by radioimmunoassay and enzyme immunoassay techniques

Maureen G. Friedman, Hava Haikin, Sophia Leventon-Kriss, Raya Joffe, Varda Goldstein, Israel Sarov

Virology

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1 Scopus citations

Abstract

An enzyme assay for the detection of antibodies to varicella-zoster membrane antigen (IPAMA) and a solid phase radioimmunoassay (RIA) which utilizes infected cell lysates as antigen are described. The results have been compared with those obtained by the indirect fluorescent antibody to membrane antigen (IFAMA) technique. It was found that IPAMA was equal in sensitivity to IFAMA. No cross-reactivity was found with other herpes group viruses. The IPAMA appears to give objective results, is easily and rapidly performed, and is recommended as a routine test for serological diagnosis of varicella-zoster infection. The RIA method is about 100 times more sensitive than IPAMA and IFAMA. The RIA is specific and has the potential of determining lower levels of antibody than other serological methods currently in use.

Original language	English
Pages (from-to)	177-186
Number of pages	10
Journal	Medical Microbiology and Immunology
Volume	166
Issue number	1-4
DOIs	https://doi.org/10.1007/BF02121148
State	Published - 1 Nov 1978

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Microbiology (medical)

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title = "Detection of antibodies to varicella zoster virus by radioimmunoassay and enzyme immunoassay techniques",

abstract = "An enzyme assay for the detection of antibodies to varicella-zoster membrane antigen (IPAMA) and a solid phase radioimmunoassay (RIA) which utilizes infected cell lysates as antigen are described. The results have been compared with those obtained by the indirect fluorescent antibody to membrane antigen (IFAMA) technique. It was found that IPAMA was equal in sensitivity to IFAMA. No cross-reactivity was found with other herpes group viruses. The IPAMA appears to give objective results, is easily and rapidly performed, and is recommended as a routine test for serological diagnosis of varicella-zoster infection. The RIA method is about 100 times more sensitive than IPAMA and IFAMA. The RIA is specific and has the potential of determining lower levels of antibody than other serological methods currently in use.",

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AU - Friedman, Maureen G.

AU - Haikin, Hava

AU - Leventon-Kriss, Sophia

AU - Joffe, Raya

AU - Goldstein, Varda

AU - Sarov, Israel

PY - 1978/11/1

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Detection of antibodies to varicella zoster virus by radioimmunoassay and enzyme immunoassay techniques

Abstract

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