Detection of galectin-3 by novel peptidic photoprobes

Monique van Scherpenzeel; Martin van der Pot; Christopher J. Arnusch; Rob M.J. Liskamp; Roland J. Pieters

doi:10.1016/j.bmcl.2006.10.043

Detection of galectin-3 by novel peptidic photoprobes

Monique van Scherpenzeel, Martin van der Pot, Christopher J. Arnusch, Rob M.J. Liskamp, Roland J. Pieters

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Photoprobes were prepared with specificity for binding, labeling, and visualizing galectin-3 in a mixture of proteins. The probes were derived from a galectin-3 binding 15-mer peptide sequence in which a benzophenone photolabel was incorporated at the N-terminus and in another case as a phenyl alanine replacement in the middle of the sequence. Detection of galectin-3 was possible in Escherichia coli lysates that were spiked with various amounts of galectin-3.

Original language	English
Pages (from-to)	376-378
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	17
Issue number	2
DOIs	https://doi.org/10.1016/j.bmcl.2006.10.043
State	Published - 15 Jan 2007
Externally published	Yes

Keywords

Galectins
Peptides
Photoprobe
Proteomics

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2006.10.043

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title = "Detection of galectin-3 by novel peptidic photoprobes",

abstract = "Photoprobes were prepared with specificity for binding, labeling, and visualizing galectin-3 in a mixture of proteins. The probes were derived from a galectin-3 binding 15-mer peptide sequence in which a benzophenone photolabel was incorporated at the N-terminus and in another case as a phenyl alanine replacement in the middle of the sequence. Detection of galectin-3 was possible in Escherichia coli lysates that were spiked with various amounts of galectin-3.",

keywords = "Galectins, Peptides, Photoprobe, Proteomics",

author = "{van Scherpenzeel}, Monique and {van der Pot}, Martin and Arnusch, {Christopher J.} and Liskamp, {Rob M.J.} and Pieters, {Roland J.}",

note = "Funding Information: We are grateful for support from the Netherlands Proteomics Centre (NPC). ",

year = "2007",

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doi = "10.1016/j.bmcl.2006.10.043",

language = "English",

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journal = "Bioorganic and Medicinal Chemistry Letters",

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AU - van Scherpenzeel, Monique

AU - van der Pot, Martin

AU - Arnusch, Christopher J.

AU - Liskamp, Rob M.J.

AU - Pieters, Roland J.

N1 - Funding Information: We are grateful for support from the Netherlands Proteomics Centre (NPC).

PY - 2007/1/15

Y1 - 2007/1/15

N2 - Photoprobes were prepared with specificity for binding, labeling, and visualizing galectin-3 in a mixture of proteins. The probes were derived from a galectin-3 binding 15-mer peptide sequence in which a benzophenone photolabel was incorporated at the N-terminus and in another case as a phenyl alanine replacement in the middle of the sequence. Detection of galectin-3 was possible in Escherichia coli lysates that were spiked with various amounts of galectin-3.

AB - Photoprobes were prepared with specificity for binding, labeling, and visualizing galectin-3 in a mixture of proteins. The probes were derived from a galectin-3 binding 15-mer peptide sequence in which a benzophenone photolabel was incorporated at the N-terminus and in another case as a phenyl alanine replacement in the middle of the sequence. Detection of galectin-3 was possible in Escherichia coli lysates that were spiked with various amounts of galectin-3.

KW - Galectins

KW - Peptides

KW - Photoprobe

KW - Proteomics

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JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 2

ER -

Detection of galectin-3 by novel peptidic photoprobes

Abstract

Keywords

ASJC Scopus subject areas

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