Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors

Evelina I. Nikelshparg; Adil A. Baizhumanov; Zhanna V. Bochkova; Sergey M. Novikov; Dmitry I. Yakubovsky; Aleksey V. Arsenin; Valentyn S. Volkov; Eugene A. Goodilin; Anna A. Semenova; Olga Sosnovtseva; Georgy V. Maksimov; Nadezda A. Brazhe

doi:10.3390/bios12010032

Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors

Evelina I. Nikelshparg, Adil A. Baizhumanov, Zhanna V. Bochkova, Sergey M. Novikov, Dmitry I. Yakubovsky, Aleksey V. Arsenin, Valentyn S. Volkov, Eugene A. Goodilin, Anna A. Semenova, Olga Sosnovtseva, Georgy V. Maksimov, Nadezda A. Brazhe

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Surface-enhanced Raman spectroscopy (SERS) is a promising tool that can be used in the detection of molecular changes triggered by disease development. Cardiovascular diseases (CVDs) are caused by multiple pathologies originating at the cellular level. The identification of these deteriorations can provide a better understanding of CVD mechanisms, and the monitoring of the identified molecular changes can be employed in the development of novel biosensor tools for early diagnostics. We applied plasmonic SERS nanosensors to assess changes in the properties of erythrocytes under normotensive and hypertensive conditions in the animal model. We found that spontaneous hypertension in rats leads (i) to a decrease in the erythrocyte plasma membrane fluidity and (ii) to a decrease in the mobility of the heme of the membrane-bound hemoglobin. We identified SERS parameters that can be used to detect pathological changes in the plasma membrane and submembrane region of erythrocytes.

Original language	English
Article number	32
Journal	Biosensors
Volume	12
Issue number	1
DOIs	https://doi.org/10.3390/bios12010032
State	Published - 1 Jan 2022
Externally published	Yes

Keywords

Biosensing
Erythrocytes
Hemoglobin
Nanoparticles
Plasma membrane
Plasmonic nanostructures
Spontaneously hypertensive rats
Surface-enhanced Raman spectroscopy

ASJC Scopus subject areas

Analytical Chemistry
Biotechnology
Biomedical Engineering
Instrumentation
Engineering (miscellaneous)
Clinical Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/bios12010032

Cite this

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title = "Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors",

abstract = "Surface-enhanced Raman spectroscopy (SERS) is a promising tool that can be used in the detection of molecular changes triggered by disease development. Cardiovascular diseases (CVDs) are caused by multiple pathologies originating at the cellular level. The identification of these deteriorations can provide a better understanding of CVD mechanisms, and the monitoring of the identified molecular changes can be employed in the development of novel biosensor tools for early diagnostics. We applied plasmonic SERS nanosensors to assess changes in the properties of erythrocytes under normotensive and hypertensive conditions in the animal model. We found that spontaneous hypertension in rats leads (i) to a decrease in the erythrocyte plasma membrane fluidity and (ii) to a decrease in the mobility of the heme of the membrane-bound hemoglobin. We identified SERS parameters that can be used to detect pathological changes in the plasma membrane and submembrane region of erythrocytes.",

keywords = "Biosensing, Erythrocytes, Hemoglobin, Nanoparticles, Plasma membrane, Plasmonic nanostructures, Spontaneously hypertensive rats, Surface-enhanced Raman spectroscopy",

author = "Nikelshparg, {Evelina I.} and Baizhumanov, {Adil A.} and Bochkova, {Zhanna V.} and Novikov, {Sergey M.} and Yakubovsky, {Dmitry I.} and Arsenin, {Aleksey V.} and Volkov, {Valentyn S.} and Goodilin, {Eugene A.} and Semenova, {Anna A.} and Olga Sosnovtseva and Maksimov, {Georgy V.} and Brazhe, {Nadezda A.}",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).",

year = "2022",

month = jan,

day = "1",

doi = "10.3390/bios12010032",

language = "English",

volume = "12",

journal = "Biosensors",

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T1 - Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors

AU - Nikelshparg, Evelina I.

AU - Baizhumanov, Adil A.

AU - Bochkova, Zhanna V.

AU - Novikov, Sergey M.

AU - Yakubovsky, Dmitry I.

AU - Arsenin, Aleksey V.

AU - Volkov, Valentyn S.

AU - Goodilin, Eugene A.

AU - Semenova, Anna A.

AU - Sosnovtseva, Olga

AU - Maksimov, Georgy V.

AU - Brazhe, Nadezda A.

N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).

PY - 2022/1/1

Y1 - 2022/1/1

N2 - Surface-enhanced Raman spectroscopy (SERS) is a promising tool that can be used in the detection of molecular changes triggered by disease development. Cardiovascular diseases (CVDs) are caused by multiple pathologies originating at the cellular level. The identification of these deteriorations can provide a better understanding of CVD mechanisms, and the monitoring of the identified molecular changes can be employed in the development of novel biosensor tools for early diagnostics. We applied plasmonic SERS nanosensors to assess changes in the properties of erythrocytes under normotensive and hypertensive conditions in the animal model. We found that spontaneous hypertension in rats leads (i) to a decrease in the erythrocyte plasma membrane fluidity and (ii) to a decrease in the mobility of the heme of the membrane-bound hemoglobin. We identified SERS parameters that can be used to detect pathological changes in the plasma membrane and submembrane region of erythrocytes.

AB - Surface-enhanced Raman spectroscopy (SERS) is a promising tool that can be used in the detection of molecular changes triggered by disease development. Cardiovascular diseases (CVDs) are caused by multiple pathologies originating at the cellular level. The identification of these deteriorations can provide a better understanding of CVD mechanisms, and the monitoring of the identified molecular changes can be employed in the development of novel biosensor tools for early diagnostics. We applied plasmonic SERS nanosensors to assess changes in the properties of erythrocytes under normotensive and hypertensive conditions in the animal model. We found that spontaneous hypertension in rats leads (i) to a decrease in the erythrocyte plasma membrane fluidity and (ii) to a decrease in the mobility of the heme of the membrane-bound hemoglobin. We identified SERS parameters that can be used to detect pathological changes in the plasma membrane and submembrane region of erythrocytes.

KW - Biosensing

KW - Erythrocytes

KW - Hemoglobin

KW - Nanoparticles

KW - Plasma membrane

KW - Plasmonic nanostructures

KW - Spontaneously hypertensive rats

KW - Surface-enhanced Raman spectroscopy

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Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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