Detection of mycoplasmas infecting cell cultures by DNA hybridization

S. Razin, M. Gross, M. Worer, Y. Pollack, G. Glaser

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Infection of cell cultures by mycoplasmas can be detected and the mycoplasma identified by Southern blot hybridization of the Eco RI-digested DNA of the suspected cell cultures with a nick-translated probe consisting of cloned ribosomal RNA genes of Mycoplasma capricolum. The probe does not hybridize with eukaryotic DNA. The hybridization pattern with mycoplasmal DNA is species specific, enabling the identification of the four most prevalent mycoplasma contaminants, Mycoplasma orale, Mycoplasma hyorhinis, Mycoplasma arginini, and Acholeplasma laidlawii. The test is also very sensitive and can detect as little as 1 ng of mycoplasmal DNA, roughly equivalent to the DNA content of 105 mycoplasmas.

Original languageEnglish
Pages (from-to)404-408
Number of pages5
JournalIn Vitro
Volume20
Issue number5
DOIs
StatePublished - 1 Jan 1984
Externally publishedYes

Keywords

  • DNA hybridization
  • cell culture infection
  • mycoplasma infection

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