Detection of Nitrosomonas spp. by polymerase chain reaction

Ali Nejidat, Aharon Abeliovich

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


A unique genomic DNA fragment was isolated from Nitrosomonas europaea ATCC 19718. Based on the sequence of this fragment, oligonucleotide primers for polymerase chain reaction amplification were prepared which amplify sequences of 775 and 658 bp. The predicted DNA fragments were both amplified from the genome of N. europaea and a Nitrosomonas spp. isolated from a local oxidation pond. The primers failed to amplify DNA from the genomes of the ammonia oxidiser Nitrosolobous multiformis, the nitrite oxidiser Nitrococcus mobilis as well as from the genomes of other unrelated heterotrophic bacteria. These DNA sequences could be amplified from 0.01 ng of N. europaea genomic DNA or from 100 intact cells, and it was possible to detect Nitrosomonas DNA in a DNA mixture extracted from water samples drawn from a local oxidation pond.

Original languageEnglish
Pages (from-to)191-194
Number of pages4
JournalFEMS Microbiology Letters
Issue number1-2
StatePublished - 1 Jul 1994


  • Nitrifying bacteria
  • Nitrosomonas europaea
  • Nitrosomonas spp
  • Oxidation pond
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


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