Detection of Plasmodium falciparum in blood using DNA hybridization

Y. Pollack, S. Metzger, R. Shemer, D. Landau, D. T. Spira, J. Golenser

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

A rapid and simple assay for detecting Plasmodium falciparum in human blood was developed. The assay is based on DNA-DNA spot hybridization, using radiolabeled P. falciparum DNA as a probe and finger prick blood as the assay sample. It is very sensitive, able to detect parasitemia levels of 0.0001% in 10 μl of blood. The assay can be quantified and used to estimate parasitemia levels. Several hundred blood samples can be processed simultaneously, and the entire procedure is completed within 24 hr. This assay can be useful for epidemiological surveys, for screening of blood by blood banks and for health authorities examining immigrants and tourists coming from malaria infested areas.

Original languageEnglish
Pages (from-to)663-667
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume34
Issue number4
DOIs
StatePublished - 1 Jan 1985
Externally publishedYes

Fingerprint

Dive into the research topics of 'Detection of Plasmodium falciparum in blood using DNA hybridization'. Together they form a unique fingerprint.

Cite this