Detection of Reactive Oxygen Species by a Carbon-Dot-Ascorbic Acid Hydrogel

Sagarika Bhattacharya, Rhitajit Sarkar, Sukhendu Nandi, Angel Porgador, Raz Jelinek

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Detection of reactive oxygen species (ROS) is important in varied biological processes, disease diagnostics, and chemotherapeutic drug screening. We constructed a ROS sensor comprising an ascorbic-acid-based hydrogel encapsulating luminescent amphiphilic carbon-dots (C-dots). The sensing mechanism is based upon ROS-induced oxidation of the ascorbic acid units within the hydrogel scaffold; as a consequence, the hydrogel framework collapses resulting in aggregation of the C-dots and quenching of their luminescence. The C-dot-hydrogel platform exhibits high sensitivity and detected ROS generated chemically in solution and in actual cell environments. We demonstrate application of the C-dot-hydrogel for evaluating the efficacy of a chemotherapeutic substance, underscoring the potential of the system for drug screening applications.

Original languageEnglish
Pages (from-to)830-836
Number of pages7
JournalAnalytical Chemistry
Volume89
Issue number1
DOIs
StatePublished - 3 Jan 2017

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